Evaluation of a nonthermal plasma needle to eliminateex vivobiofilms in root canals of extracted human teeth

Autor: Anthony Nguyen, J. W. Costerton, Marcelo Freire, Christoph Schaudinn, Paul Webster, Chunqi Jiang, D. Jaramillo, Parish P. Sedghizadeh
Rok vydání: 2013
Předmět:
Zdroj: International Endodontic Journal. 46:930-937
ISSN: 0143-2885
DOI: 10.1111/iej.12083
Popis: Schaudinn C , Jaramillo D , Freire MO , SedghizadehPP , Nguyen A , Webster P , Costerton JW , Jiang C. Evaluation of a nonthermal plasma needle to eliminateex vivo biofilms in root canals of extracted human teeth.International Endodontic Journal. Aim To evaluate the efficacy of a nonthermal plasma(NTP) at atmospheric pressure on ex vivo biofilm inroot canals of extracted teeth.Methodology Intracanal contents from three teethwith root canal infections were collected, pooled andgrown in thirty-five microCT-mapped root canals ofextracted and instrumented human teeth. One groupof teeth was treated with NTP, another with 6%NaOCl and one set was left untreated. The intracanalcontents from twenty-seven teeth (nine teeth in eachgroup) were plated on agar and colony forming unitswere determined. Parametric test of one-way analysisof variance ( ANOVA ) was used to analyse statisticalsignificance. The remaining teeth were cut open,stained with LIVE/DEAD and examined with confo-cal laser scanning microscopy.Results The untreated root canals were coveredwith biofilm of varying thickness. Treatment withnonthermal plasma decreased the number of viablebacteria in biofilms by one order of magnitude, whilstthe NaOCl control achieved a reduction of more thanfour magnitudes. Both the NTP and the NaOCl treat-ment results were significantly different from the neg-ative control (P < 0.05).Conclusion The nonthermal plasma displayed anti-microbial activity against endodontic biofilms in rootcanals, but was not as effective as the use of 6%NaOCl.Keywords: biofilm, Endodontics, micro-CT, non-thermal plasma.
Databáze: OpenAIRE
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