The interaction between the archaeal elongation factor 1alpha and its nucleotide exchange factor 1beta
Autor: | Vincenzo Bocchini, Gennaro Raimo, Mariorosario Masullo |
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Rok vydání: | 1999 |
Předmět: |
Time Factors
GTP' Stereochemistry ved/biology.organism_classification_rank.species Biophysics Elongation factor 1β Biochemistry Catalysis Protein–protein interaction Sulfolobus Nucleotide exchange factor Elongation factor 1b Exchange factor Sulfolobus solfataricus Protein-protein interaction Archaea Structural Biology Genetics Guanine Nucleotide Exchange Factors Nucleotide Molecular Biology chemistry.chemical_classification biology ved/biology Temperature Proteins Cell Biology biology.organism_classification Elongation factor Kinetics chemistry Protein Binding |
Zdroj: | FEBS letters. 451(2) |
ISSN: | 0014-5793 |
Popis: | In Sulfolobus solfataricus the binding of the exchange factor 1beta (SsEF-1beta) to SsEF-1alpha-GDP displaces the nucleotide and the SsEF-1alpha-SsEF-1beta complex is formed. The complex itself is stable, but it dissociates upon the addition of GDP or Gpp(NH)p but not ATP. Since the rate of the formation of the SsEF-1alpha-SsEF-1beta complex is significatively slower than the rate of the nucleotide exchange catalyzed by SsEF-1beta it can be inferred that in vivo the GDP/GTP exchange reaction proceeds via an SsEF-1alpha-SsEF-1beta interaction without involving the formation of a stable binary complex as an intermediate. |
Databáze: | OpenAIRE |
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