A HILIC-UHPLC–MS/MS untargeted urinary metabonomics combined with quantitative analysis of five polar biomarkers on osteoporosis rats after oral administration of Gushudan
| Autor: | Zhili Xiong, Jinghan Sun, Feng Qin, Longshan Zhao, Yongqing Wen, Xiao Wu, Yue Huang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Taurine Clinical Biochemistry Administration Oral Urine 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Betaine Limit of Detection Tandem Mass Spectrometry Animals Metabolomics Rats Wistar Chromatography High Pressure Liquid Hypoxanthine Detection limit Principal Component Analysis Creatinine Chromatography Chemistry Hydrophilic interaction chromatography 010401 analytical chemistry Reproducibility of Results Cell Biology General Medicine Rats 0104 chemical sciences 030104 developmental biology Linear Models Metabolome Quantitative analysis (chemistry) Biomarkers Drugs Chinese Herbal |
| Zdroj: | Journal of Chromatography B. 1072:40-49 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2017.10.005 |
| Popis: | A HILIC-UHPLC-MS/MS untargeted urinary metabonomic method combined with quantitative analysis of five potential polar biomarkers in rat urine was developed and validated, to further understand the anti-osteoporosis effect of Gushudan(GSD) and its mechanism on prednisolone-induced osteoporosis(OP) rats in this study. The metabolites were separated and identified on Waters BEH HILIC (2.1mm×100mm, 1.7μm) column using the Waters ACQUITY™ ultra performance liquid chromatography system (Waters Corporation, Milford, USA) coupled with a Micromass Quattro Micro™ API mass spectrometer (Waters Corp, Milford, MA, USA). Principal component analysis (PCA) was used to identify potential biomarkers. Primary potential polar biomarkers including creatinine, taurine, betaine, hypoxanthine and cytosine, which were related to energy metabolism, lipid metabolism and amino acid metabolism, were found in the untargeted metabonomic research. Moreover, these targeted biomarkers were further separated and quantified in multiple-reaction monitoring (MRM) with positive ionization mode, using tinidazole as internal standard (I.S.). Good linearities (r>0.99) were obtained for all the analytes with the low limit of quantification from 1.00 to 12.8μg/mL. The relative standard deviation (RSD) of the intra-day and inter-day precisions were within 15.0% and the accuracy ranged from -14.3% to 13.5%. The recovery was more than 85.0%. And the validated method was successfully applied to investigate the urine samples of the control group, prednisolone-induced osteoporosis model group and Gushudan-treatment group in rats. Compared to the control group, the level of creatinine, taurine, betaine, hypoxanthine and cytosine in the model group revealed a significant decrease trend (p |
| Databáze: | OpenAIRE |
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