Gene gun particle-mediated vaccination with plasmid DNA confers protective immunity against rabies virus infection
| Autor: | Larry C. Ewalt, Donald L. Lodmell, Nancy B. Ray |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Time Factors
Rabies Antibodies Viral medicine.disease_cause Virus Gene gun Mice Viral Envelope Proteins Vaccines DNA medicine Virus-neutralizing Antibody Animals Neutralizing antibody Mononegavirales Antigens Viral Lyssavirus Glycoproteins General Veterinary General Immunology and Microbiology biology Vaccination Rabies virus Public Health Environmental and Occupational Health Viral Vaccines Biolistics biology.organism_classification medicine.disease Virology Infectious Diseases DNA Viral biology.protein Molecular Medicine Female |
| Zdroj: | Vaccine. 16:115-118 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(97)88325-9 |
| Popis: | Accell gene gun particle-mediated immunization with DNA encoding the glycoprotein gene of the challenge virus standard strain of rabies virus was evaluated for its ability to elicit protective levels of serum anti-rabies virus neutralizing antibody. Strong primary and booster neutralizing antibody responses were detected in mice following immunization with 2 micrograms of DNA coated on 2.6-micron gold beads. Protective levels of antibody persisted for over 300 days. Mice challenged intraplantarly 315 days post-primary immunization (225 days post-booster vaccination) survived lethal rabies virus challenge. Our data demonstrate a potentially significant role for gene gun-based delivery of DNA in the field of rabies virus vaccination. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect