Expression of P-glycoprotein in the intestinal epithelium of dogs with lymphoplasmacytic enteritis
| Autor: | Sylvie Daminet, S. Van der Heyden, Stijn Schauvliege, B. Wegge, Dominique Paepe, Koen Chiers, Richard Ducatelle, Ingeborgh Polis, Griet Vercauteren, Myriam Hesta, Tim Waelbers |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Pathology Biopsy Ileum Biology Gastroenterology Pathology and Forensic Medicine Enteritis Jejunum Dogs Internal medicine medicine Animals Large intestine ATP Binding Cassette Transporter Subfamily B Member 1 Dog Diseases Duodenal Diseases Intestinal Mucosa General Veterinary Apical membrane medicine.disease Inflammatory Bowel Diseases Intestinal epithelium Immunohistochemistry Small intestine medicine.anatomical_structure Duodenum |
| Zdroj: | Journal of comparative pathology. 145(2-3) |
| ISSN: | 1532-3129 |
| Popis: | Inflammatory bowel disease (IBD) is an idiopathic chronic inflammatory disease of the stomach, the small intestine and/or the large intestine. Loss of integrity of the intestinal barrier may be an important factor in the pathogenesis of IBD. In dogs, lymphoplasmacytic enteritis (LPE) is one of the recognized forms of IBD. P-glycoprotein (P-gp) is a membrane-bound efflux pump constituting an important component of the intestinal barrier. Changes in P-gp expression at the level of the intestinal barrier may be important in the pathogenesis of canine LPE, as this may lead to variable protection against xenobiotics and bacterial products in the intestine. The aim of the present study was to evaluate the expression of epithelial P-gp in the intestine in dogs with LPE compared with disease-free animals. Formalin-fixed intestinal biopsy samples from 57 dogs with histopathological evidence of LPE were immunolabelled with anti-P-gp antibodies (C494 and C219). Endoscopic biopsy samples of the duodenum and colon from 16 healthy beagles were used as controls. None of the control dogs had P-gp expression in the apical membrane of duodenal enterocytes, but all had P-gp labelling at the colonic epithelial surface. Twenty out of 57 dogs with LPE had P-gp expression at the apical surface membrane of villus epithelial cells in the duodenum, jejunum and/or ileum. Six out of 16 colonic samples from dogs with LPE had decreased P-gp expression at the epithelial surface compared with controls. It is unclear whether these changes in P-gp expression in dogs with LPE are a cause or a consequence of the inflammation. The observed changes could affect bioavailability of therapeutic drugs used in LPE. |
| Databáze: | OpenAIRE |
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