Arabidopsis cell wall composition determines disease resistance specificity and fitness

Autor: Sivakumar Pattathil, Tinguaro Rodriguez, Amandine Freydier, Andrea Sánchez-Vallet, Gemma López, Deborah Goffner, Michael G. Hahn, Antonio Molina, Eva Miedes, Nicolas Denancé, Marie-Pierre Rivière, Laura Bacete, Hugo Mélida, Xavier Barlet
Přispěvatelé: Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agence Nationale de la Recherche (France), Agencia Estatal de Investigación (España), European Commission, Molina, Antonio [0000-0003-3137-7938], Miedes, Eva [0000-0003-2899-1494], Bacete, Laura [0000-0003-3171-8181], Rodríguez, Tinguaro [0000-0003-4989-2348], Mélida, Hugo [0000-0003-1792-0113], Denancé, Nicolas [0000-0003-0173-3970], Sánchez-Vallet, Andrea [0000-0002-3668-9503], Rivière, Marie-Pierre [0000-0003-0504-9797], Barlet, Xavier [0000-0002-9899-6859], Pattathil, Sivakumar [0000-0003-3870-4137], Hahn, Michael [0000-0003-2136-5191], Goffner, Deborah [0000-0002-1232-7911], Universidad Politécnica de Madrid (UPM), Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Universidad de León [León], Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), GEVES Station nationale d'essais de semences, Institut National de la Recherche Agronomique (INRA), Evotec ID [Lyon], University System of Georgia (USG), National Science Foundation (NSF) DBI-0421683 et IOS 0923992, Spanish Ministry of Economy and Competitiveness (MINECO) of Spanish Ministry of Science, Innovation, and Universities (MICIU) : BIO2015-64077-R, Spanish Ministry of Economy and Competitiveness (MINECO) of Spanish Ministry of Science, Innovation, and Universities (MICIU) : RTI2018-096975-B-I00, Severo Ochoa Program for Centers of Excellence in R&D from the Agencia Estatal de Investigacion of Spain : SEV-2016-0672, ANR-07-GPLA-0014,WALLTALK,Plant cell walls : where microbes meet plants(2007), European Project: 624721,EC:FP7:PEOPLE,FP7-PEOPLE-2013-IEF,SIGNWALLING(2015), Molina, Antonio, Miedes, Eva, Bacete, Laura, Rodríguez, Tinguaro, Mélida, Hugo, Denancé, Nicolas, Sánchez-Vallet, Andrea, Rivière, Marie-Pierre, Barlet, Xavier, Pattathil, Sivakumar, Hahn, Michael, Goffner, Deborah, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Sophia Antipolis, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)
Rok vydání: 2021
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2021, 118 (5), 12 p. ⟨10.1073/pnas.2010243118⟩
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2021, 118 (5), 12 p. ⟨10.1073/pnas.2010243118⟩
ISSN: 0027-8424
1091-6490
Popis: Departamento de Biotecnología (INIA)
Plant cell walls are complex structures subject to dynamic remodeling in response to developmental and environmental cues and play essential functions in disease resistance responses. We tested the specific contribution of plant cell walls to immunity by determining the susceptibility of a set of Arabidopsis cell wall mutants (cwm) to pathogens with different parasitic styles: a vascular bacterium, a necrotrophic fungus, and a biotrophic oomycete. Remarkably, most cwm mutants tested (29/34; 85.3%) showed alterations in their resistance responses to at least one of these pathogens in comparison to wild-type plants, illustrating the relevance of wall composition in determining disease-resistance phenotypes. We found that the enhanced resistance of cwm plants to the necrotrophic and vascular pathogens negatively impacted cwm fitness traits, such as biomass and seed yield. Enhanced resistance of cwm plants is not only mediated by canonical immune pathways, like those modulated by phytohormones or microbe-associated molecular patterns, which are not deregulated in the cwm tested. Pectin-enriched wall fractions isolated from cwm plants triggered immune responses in wild-type plants, suggesting that wall-mediated defensive pathways might contribute to cwm resistance. Cell walls of cwm plants show a high diversity of composition alterations as revealed by glycome profiling that detect specific wall carbohydrate moieties. Mathematical analysis of glycome profiling data identified correlations between the amounts of specific wall carbohydrate moieties and disease resistance phenotypes of cwm plants. These data support the relevant and specific function of plant wall composition in plant immune response modulation and in balancing disease resistance/development trade-offs.
This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) grants BIO2015-64077-R and RTI2018-096975-B-I00 of Spanish Ministry of Science, Innovation, and Universities (MICIU) to A.M. and by the French National Agency for Research Grant ANR-07-GPLA-014 to D.G. This work has been also financially supported by the Severo Ochoa Program for Centers of Excellence in R&D from the Agencia Estatal de Investigación of Spain (Grant SEV-2016-0672 (2017-2021) to the Centro de Biotecnología y Genómica de Plantas). In the frame of this program, H.M. was a postdoctoral fellow. H.M. was also supported by an Individual Fellowship grant (SignWALLINg-624721) from the European Union. E.M. was a Juan de la Cierva Postdoctoral Fellow from MINECO, and L.B. was a Formacion Personal Investigador fellow of MICIU.
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Databáze: OpenAIRE
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