Expression of interferon-? receptors on bladder cancer cells: does it correlate with biological response?
| Autor: | R. A. Hawkins, Geoffrey D. Chisholm, John F. Smyth, S. J. Hawkyard, Andrew Jackson, Keith James |
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| Rok vydání: | 1992 |
| Předmět: |
Carcinoma
Transitional Cell Bladder cancer Clinical chemistry Urology Cell Significant difference Human bladder Biology medicine.disease Molecular biology Cell Line Interferon-gamma Kinetics medicine.anatomical_structure Urinary Bladder Neoplasms Cell culture Tumor Cells Cultured medicine Humans Interferon gamma Receptors Immunologic Receptor Receptors Interferon medicine.drug |
| Zdroj: | Urological Research. 20:229-232 |
| ISSN: | 1434-0879 0300-5623 |
| DOI: | 10.1007/bf00299722 |
| Popis: | Previously we have shown a differential biological response of three human bladder cancer cell lines (RT4, RT112 and MGH-U1) to gamma interferon (IFN-gamma). The present study examines the relationship between the biological response and the expression of the interferon-gamma receptor on the tumour cell surface. Using a competitive radioligand binding assay and Scatchard analysis, we measured the number and affinity of the IFN-gamma receptors on each of the above cell lines. Individual cells from each line expressed large numbers (29,100-41,800) of high-affinity receptors (kd = 2.4-3.9 x 10(10) M). There was no statistically significant difference in either of these parameters between the three lines. We therefore conclude that the biological response of these bladder lines to IFN-gamma does not relate to the number or affinity of its receptor on the plasma membrane of these tumour cells. |
| Databáze: | OpenAIRE |
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