Non-selective Separation of Bacterial Cells with Magnetic Nanoparticles Facilitated by Varying Surface Charge
| Autor: | Ji Li, Ming-Fei Shao, Xin-Lei Gao, Yi Luo, Yi-Sheng Xu, Kai Zhang, Feng Ouyang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) surface charge Microorganism Indoor bioaerosol lcsh:QR1-502 magnetic nanoparticle Nanoparticle 010501 environmental sciences 01 natural sciences Microbiology lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound Adsorption 0105 earth and related environmental sciences Original Research biology Ecology biology.organism_classification glass fiber filter 030104 developmental biology Chemical engineering Microbial population biology chemistry bioaerosols adsorption Magnetic nanoparticles microbial community Citric acid Bacteria |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 7 (2016) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2016.01891 |
| Popis: | Recovering microorganisms from environmental samples is a crucial primary step for understanding microbial communities using molecular ecological approaches. It is often challenging to harvest microorganisms both efficiently and unselectively, guaranteeing a similar microbial composition between original and separated biomasses. A magnetic nanoparticles (MNPs) based method was developed to effectively separate microbial biomass from glass fiber pulp entrapped bacteria. Buffering pH and nanoparticle silica encapsulation significantly affected both biomass recovery and microbial selectivity. Under optimized conditions (using citric acid coated Fe3O4, buffering pH = 2.2), the method was applied in the pretreatment of total suspended particle sampler collected bioaerosols, the effective volume for DNA extraction was increased 10-folds, and the overall method detection limit of microbial contaminants in bioaerosols significantly decreased. A consistent recovery of the majority of airborne bacterial populations was demonstrated by in-depth comparison of microbial composition using 16S rRNA gene high-throughput sequencing. Surface charge was shown as the deciding factor for the interaction between MNPs and microorganisms, which helps developing materials with high microbial selectivity. To our knowledge, this study is the first report using MNPs to separate diverse microbial community unselectively from a complex environmental matrix. The technique is convenient and sensitive, as well as feasible to apply in monitoring of microbial transport and other related fields. |
| Databáze: | OpenAIRE |
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