Esophageal 3D Culture Systems as Modeling Tools in Esophageal Epithelial Pathobiology and Personalized Medicine
| Autor: | Hiroshi Nakagawa, Kelly A. Whelan, Amanda B. Muir |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Organoid
0301 basic medicine Basic science Spheroid Culture Stratified squamous epithelium Context (language use) Review Esophageal Disease STAT3 signal transducer and activator of transcription-3 03 medical and health sciences BE Barrett’s esophagus 3D 3-dimensional medicine GERD gastroesophageal reflux disease EoE eosinophilic esophagitis FEF3 primary human fetal esophageal fibroblast lcsh:RC799-869 Esophagus Eosinophilic esophagitis EGF epidermal growth factor Hepatology Esophageal disease business.industry Organotypic Culture Gastroenterology medicine.disease CSC cancer stem cell EGFR epidermal growth factor receptor 3. Good health COX cyclooxygenase OTC organotypic 3-dimensional culture 030104 developmental biology medicine.anatomical_structure Cancer research Adenocarcinoma lcsh:Diseases of the digestive system. Gastroenterology Personalized medicine ESCC esophageal squamous cell carcinoma EMT epithelial-mesenchymal transition business EADC esophageal adenocarcinoma |
| Zdroj: | Cellular and Molecular Gastroenterology and Hepatology, Vol 5, Iss 4, Pp 461-478 (2018) Cellular and Molecular Gastroenterology and Hepatology |
| ISSN: | 2352-345X |
| Popis: | The stratified squamous epithelium of the esophagus shows a proliferative basal layer of keratinocytes that undergo terminal differentiation in overlying suprabasal layers. Esophageal pathologies, including eosinophilic esophagitis, gastroesophageal reflux disease, Barrett's esophagus, squamous cell carcinoma, and adenocarcinoma, cause perturbations in the esophageal epithelial proliferation-differentiation gradient. Three-dimensional (3D) culture platforms mimicking in vivo esophageal epithelial tissue architecture ex vivo have emerged as powerful experimental tools for the investigation of esophageal biology in the context of homeostasis and pathology. Herein, we describe types of 3D culture that are used to model the esophagus, including organotypic, organoid, and spheroid culture systems. We discuss the development and optimization of various esophageal 3D culture models; highlight the applications, strengths, and limitations of each method; and summarize how these models have been used to evaluate the esophagus under homeostatic conditions as well as under the duress of inflammation and precancerous/cancerous conditions. Finally, we present future perspectives regarding the use of esophageal 3D models in basic science research as well as translational studies with the potential for personalized medicine. Keywords: Organotypic Culture, Organoid, Spheroid Culture, Esophageal Disease |
| Databáze: | OpenAIRE |
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