IFNγ deficient C57BL/6 (H-2b) mice develop collagen induced arthritis with predominant usage of T cell receptor Vß6 and Vß8 in arthritic joints
| Autor: | Chu Cq, Paul H. Wooley, Zheng Song, Lois Mayton, Bin Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
musculoskeletal diseases
Cellular immunity T cell Receptors Antigen T-Cell alpha-beta Immunology Arthritis General Biochemistry Genetics and Molecular Biology Interferon-gamma Mice Rheumatology medicine Immunology and Allergy Animals Interferon gamma RNA Messenger Mice Knockout Chi-Square Distribution business.industry Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha T-cell receptor Interleukin medicine.disease Arthritis Experimental Extended Report Mice Inbred C57BL medicine.anatomical_structure Mice Inbred DBA Rheumatoid arthritis Models Animal Tumor necrosis factor alpha Female Joints Collagen Disease Susceptibility business medicine.drug Interleukin-1 |
| Popis: | Transgenic deficiency in interferon gamma (IFNgamma) or IFNgamma receptor makes resistant strains of mice bearing H-2(b) or H-2(d) susceptible to collagen induced arthritis (CIA).To determine whether the escape from regulation of disease susceptibility at the major histocompatibility complex level involves a new use of autoimmune T cells expressing T cell receptor (TCR) Vbeta that vary from the cell populations previously identified within arthritic joints.Arthritis was induced by a standard protocol with type II bovine collagen (CII) in complete Freund's adjuvant. Clinical features, histopathology, immunological responses, and TCR profile in arthritic joints in IFNgamma knockout C57BL/6 (B6.IFNgamma KO) mice (H-2(b)) were compared directly with those in DBA/1 mice (H-2(q)).60-80% of B6.IFNgamma KO mice developed a progressive arthritis with a similar clinical course to classical CIA in DBA/1 mice. The affected joints in B6.IFNgamma KO mice had an erosive form of arthritis with similar features to joint disease in DBA/1 mice. B6.IFNgamma KO mice produced significantly higher levels of IgG2b and IgG1 autoantibodies to murine CII and showed increased proliferative response to CII compared with B6 mice. Comparable levels of interleukin 1beta and tumour necrosis factor alpha expression were detected in arthritic joints from beta6.IFNgamma KO and DBA/1 mice. B6.IFNgammaKO mice used predominantly TCR Vbeta6 and Vbeta8 in arthritic joints. This TCR Vbeta profile is similar to that found in DBA/1 mice with CIA.C57BL/6 mice deficient in IFNgamma production can develop arthritis that resembles classical CIA. These data suggest that IFNgamma is a key factor mediating susceptibility to CIA. |
| Databáze: | OpenAIRE |
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