Functional variability of the Lr34 durable resistance gene in transgenic wheat

Autor:	Gabriele Buesing, Simon G. Krattinger, Terese Richardson, Liselotte L. Selter, Evans Lagudah, Libby Viccars, Beat Keller, Joanna M. Risk, Gerhard Herren
Přispěvatelé:	University of Zurich, Lagudah, Evans S
Rok vydání:	2012
Předmět:	0106 biological sciences Hypersensitive response Transgene Molecular Sequence Data Plant Science 580 Plants (Botany) Plant disease resistance Biology Genes Plant 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 10126 Department of Plant and Microbial Biology Gene Expression Regulation Plant 1110 Plant Science Botany Plant Immunity 1102 Agronomy and Crop Science Allele Gene Triticum 030304 developmental biology Plant Diseases Plant Proteins 2. Zero hunger Genetics Regulation of gene expression 0303 health sciences Basidiomycota Callose food and beverages Plants Genetically Modified Genetically modified organism Cold Temperature Plant Leaves chemistry Seedlings 1305 Biotechnology Agronomy and Crop Science 010606 plant biology & botany Biotechnology
Zdroj:	Plant Biotechnology Journal
ISSN:	1467-7652
Popis:	Breeding for durable disease resistance is challenging, yet essential to improve crops for sustainable agriculture. The wheat Lr34 gene is one of the few cloned, durable resistance genes in plants. It encodes an ATP binding cassette transporter and has been a source of resistance against biotrophic pathogens, such as leaf rust (Puccinina triticina), for over 100 years. As endogenous Lr34 confers quantitative resistance, we wanted to determine the effects of transgenic Lr34 with specific reference to how expression levels affect resistance. Transgenic Lr34 wheat lines were made in two different, susceptible genetic backgrounds. We found that the introduction of the Lr34 resistance allele was sufficient to provide comparable levels of leaf rust resistance as the endogenous Lr34 gene. As with the endogenous gene, we observed resistance in seedlings after cold treatment and in flag leaves of adult plants, as well as Lr34-associated leaf tip necrosis. The transgene-based Lr34 resistance did not involve a hypersensitive response, altered callose deposition or up-regulation of PR genes. Higher expression levels compared to endogenous Lr34 were observed in the transgenic lines both at seedling as well as adult stage and some improvement of resistance was seen in the flag leaf. Interestingly, in one genetic background the transgenic Lr34-based resistance resulted in improved seedling resistance without cold treatment. These data indicate that functional variability in Lr34-based resistance can be created using a transgenic approach.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51b4a2f58916be78ef1676928e412517 https://pubmed.ncbi.nlm.nih.gov/22321563 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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