Cathepsin D in prawn reproductive system: its localization and function in actin degradation
| Autor: | Wattana Weerachatyanukul, Piyaporn Surinlert, Chompoonut Sukonset, Jirasuda Nakeim, Orawan Thongsum, Monsicha Somrit, Atthaboon Watthammawut, Somluk Asuvapongpatana |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
endocrine system
Somatic cell lcsh:Medicine Cathepsin D Acrosomal matrix Filamentous actin General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Testis medicine Agricultural Science Spermatogenesis Molecular Biology Actin 030304 developmental biology 0303 health sciences Chemistry General Neuroscience lcsh:R 030302 biochemistry & molecular biology Actin meshwork General Medicine Sperm Epithelium Cell biology medicine.anatomical_structure Aquaculture Fisheries and Fish Science CAT-D General Agricultural and Biological Sciences Zoology |
| Zdroj: | PeerJ, Vol 8, p e10218 (2020) PeerJ |
| ISSN: | 2167-8359 |
| DOI: | 10.7717/peerj.10218 |
| Popis: | Cathepsin D (CAT-D) is a well-known aspartic protease that serves a function as house-keeping lysosomal enzyme in all somatic cells. Its existence in reproductive tissues is highly variable, even in the somatic derived epithelial cells of reproductive tract. InMacrobrachium rosenbergii, existence of MrCAT-D and its translational product was detected in both somatic cells (Sertoli-like supporting cells) and developing spermatogenic cells as well as along accessory spermatic ducts. Specifically, MrCAT-D was localized onto the sperm surface rather than within the acrosomal matrix, as evident by similar staining pattern of anti-CAT-D on live and aldehyde fixed sperm. MrCAT-D in testicular extracts and sperm isolates showed active enzyme activities towards its specific fluorogenic substrate (MCA-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys (Dnp)-D-Arg-NH2). MrCAT-D also exerted its function towards hydrolyzing filamentous actin, the meshwork of which is shown to be localized at the junction between germ cells and supporting cells and spermatogonia inM. rosenbergiitesticular epithelium. Together, we have localized MrCAT-D transcript and its translational product in both supporting and germ cells of testis and claimed its enzymatic function towards actin degradation, which may be related to sperm release from the epithelial cell interaction. |
| Databáze: | OpenAIRE |
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