Daucosterol promotes the proliferation of neural stem cells
| Autor: | Mingyan Wang, Feng-ming Zhao, Jianping Chen, Nian-yun Yang, Li-hua Jiang, Yi-jie Zou, Da-xiang Lu, Xiao-lin Yuan |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Endocrinology
Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Basic fibroblast growth factor Biology Carboxyfluorescein diacetate succinimidyl ester Biochemistry chemistry.chemical_compound Endocrinology Mitotic cell cycle Neural Stem Cells Epidermal growth factor medicine Animals RNA Messenger Insulin-Like Growth Factor I Molecular Biology Cell Proliferation Epidermal Growth Factor Growth factor Cell Differentiation Cell Biology Cell cycle Daucosterol Sitosterols Molecular biology Neural stem cell Rats chemistry Molecular Medicine Fibroblast Growth Factor 2 Proto-Oncogene Proteins c-akt |
| Zdroj: | The Journal of Steroid Biochemistry and Molecular Biology. 140:90-99 |
| ISSN: | 0960-0760 |
| Popis: | Neural stem cells (NSCs) are self-regenerating cells, but their regenerative capacity is limited. The present study was conducted to investigate the effect of daucosterol (a sterolin) on the promotion of NSC proliferation and determine the corresponding molecular mechanism. Results of cell counting kit-8 (CCK-8) assay showed that daucosterol significantly increased the quantity of viable cells and the effectiveness of daucosterol was similar to that of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Flow cytometry detection of CFSE-labeled (CFSE, carboxyfluorescein diacetate succinimidyl ester) NSCs showed that Div Index (or the average number of cell divisions) and % Divided (or the percentage of cells that divided at least once) of the cells were increased, indicating that daucosterol increased the percentage of NSCs re-entering the cell cycle. mRNA microarray analysis showed that 333 genes that are mostly involved in the mitotic cell cycle were up-regulated. By contrast, 627 genes that are mostly involved in differentiation were down-regulated. In particular, insulin-like growth factor I (IGF1) was considered as an important regulatory gene that functionally promoted NSC proliferation, and the increased expression of IGF1 protein was validated by ELISA. In addition, the phosphorylation of AKT was increased, indicating that the proliferation-enhancing activity of daucosterol may be involved in IGF1-AKT pathway. Our study provided information about daucosterol as an efficient and inexpensive growth factor alternative that could be used in clinical medicine and research applications. |
| Databáze: | OpenAIRE |
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