Development of mouse monoclonal antibody for detecting hemagglutinin of avian influenza A(H7N9) virus and preventing virus infection

Autor: Shih-Chung Chang, Heng-Yi Su, Chia-Wei Weng, Hui-Wen Chen, Yi-Wei Chiang, Chia-Jung Li, Kai-Ting Hsieh
Rok vydání: 2021
Předmět:
Monoclonal antibody
Hemagglutination
Neutralization activity
medicine.drug_class
Influenza A Virus
H7N7 Subtype
Hemagglutinin (influenza)
Hemagglutinin Glycoproteins
Influenza Virus
Hemagglutination inhibition
Biology
Antibodies
Viral
Influenza A Virus
H7N9 Subtype
medicine.disease_cause
Applied Microbiology and Biotechnology
Cross-reactivity
Virus
Epitope
Mice
03 medical and health sciences
Influenza A Virus
H1N1 Subtype
Influenza
Human
medicine
Animals
Humans
Hemagglutinin
Avian influenza A(H7N9) virus
Applied Genetics and Molecular Biotechnology
030304 developmental biology
0303 health sciences
Hemagglutination assay
Influenza A Virus
H5N1 Subtype
030306 microbiology
Influenza A Virus
H3N2 Subtype
Antibodies
Monoclonal
General Medicine
Virology
Influenza A virus subtype H5N1
Trypsinization prevention
Hemagglutinins
Lateral flow immunochromatographic test
biology.protein
Biotechnology
Zdroj: Applied Microbiology and Biotechnology
ISSN: 1432-0614
0175-7598
DOI: 10.1007/s00253-021-11253-7
Popis: Many cases of avian influenza A(H7N9) virus infection in humans have been reported since its first emergence in 2013. The disease is of concern because most patients have become severely ill with roughly 30% mortality rate. Because the threat in public health caused by H7N9 virus remains high, advance preparedness is essentially needed. In this study, the recombinant H7N9 hemagglutinin (HA) was expressed in insect cells and purified for generation of two monoclonal antibodies, named F3-2 and 1C6B. F3-2 can only recognize the H7N9 HA without having cross-reactivity with HA proteins of H1N1, H3N2, H5N1, and H7N7. 1C6B has the similar specificity with F3-2, but 1C6B can also bind to H7N7 HA. The binding epitope of F3-2 is mainly located in the region of H7N9 HA(299–307). The binding epitope of 1C6B is located in the region of H7N9 HA(489–506). F3-2 and 1C6B could not effectively inhibit the hemagglutination activity of H7N9 HA. However, F3-2 can prevent H7N9 HA from trypsin cleavage and can bind to H7N9 HA which has undergone pH-induced conformational change. F3-2 also has the ability of binding to H7N9 viral particles and inhibiting H7N9 virus infection to MDCK cells with the IC50 value of 22.18 μg/mL. In addition, F3-2 and 1C6B were utilized for comprising a lateral flow immunochromatographic test strip for specific detection of H7N9 HA. Key points • Two mouse monoclonal antibodies, F3-2 and 1C6B, were generated for recognizing the novel binding epitopes in H7N9 HA. • F3-2 can prevent H7N9 HA from trypsin cleavage and inhibit H7N9 virus infection to MDCK cells. • F3-2 and 1C6B were developed as a lateral flow immunochromatographic test for specific detection of H7N9 HA.
Databáze: OpenAIRE
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