THYMIDINE SUICIDE IN VIVO AND IN VITRO OF SPLEEN COLONY FORMING AND AGAR COLONY FORMING CELLS OF MOUSE BONE MARROW
| Autor: | R E Millard, H M Belcher, N M Blackett |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Cell Survival
Bone Marrow Cells Cell Count Spleen Biology Tritium Mice Tissue culture chemistry.chemical_compound Bone Marrow In vivo Culture Techniques Cesium Isotopes medicine Animals Transplantation Homologous Progenitor cell Bone Marrow Transplantation Radioisotopes DNA Cell Biology General Medicine Molecular biology In vitro Clone Cells Mice Inbred C57BL Radiation Effects Transplantation Agar medicine.anatomical_structure chemistry Immunology Mice Inbred CBA Bone marrow Thymidine Cell Division |
| Zdroj: | Cell Proliferation. 7:309-318 |
| ISSN: | 1365-2184 0960-7722 |
| DOI: | 10.1111/j.1365-2184.1974.tb00413.x |
| Popis: | The ‘thymidine suicide’technique for indicating differences in the proliferation rate of early haemopoietic progenitor cells (spleen colony forming and agar colony forming cells) in C57BL mice has been evaluated. Special care was taken to use the same bone marrow cell suspension for the two progenitor cell assays. Both the in vivo and the in vitro techniques were employed. Following 3H-TdR in vivo, about 20% of both types of progenitor cell are killed in normal mice; however, after incubation in vitro with 3H-TdR, 35% of agar colony forming cells but only 4% of spleen colony forming cells are killed. Reasons for the difference between the in vivo and the in vitro results are discussed. With bone marrow from continuously irradiated animals, the thymidine suicide for both agar colony forming and spleen colony forming cells is in the range 42–50%, and there is no difference between in vivo and in vitro suicide. The in vivo results support the conclusion, based on the effect of proliferation dependent cytotoxic agents, that in C57BL mice agar colony forming and spleen colony forming cells are proliferating at the same rate in normal animals, and are speeded up to the same extent by continuous γ-irradiation. It is considered that in normal C57BL mice the in vitro method does not give a correct estimate of the proliferation rate of these progenitor cells. It would seem that the similarity in the proliferation rate of agar colony forming and spleen colony forming cells in C57BL mice is not true for other strains of mice: indeed using normal CBA and in vivo suicide, we have shown a significantly greater thymidine suicide for agar colony forming cells compared to spleen colony forming cells. |
| Databáze: | OpenAIRE |
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