Tissue proteome profiling of preeclamptic placenta using recombinant antibody microarrays
| Autor: | Magnus Centlow, Carl A.K. Borrebaeck, Sara Nygren, Christer Wingren, Stefan R. Hansson, Linda Dexlin-Mellby, Anna Sandström |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Microarray Proteome Placenta Clinical Biochemistry Protein Array Analysis Gestational Age Biology Bioinformatics Proteomics Preeclampsia Young Adult Pre-Eclampsia Pregnancy medicine Humans reproductive and urinary physiology Gene Expression Profiling Middle Aged medicine.disease Recombinant Proteins medicine.anatomical_structure Membrane protein embryonic structures Biomarker (medicine) Female Pregnancy disorder |
| Zdroj: | Proteomics. Clinical applications. 4(10-11) |
| ISSN: | 1862-8354 |
| Popis: | Purpose: Preeclampsia (PE) is a severe, multi-system pregnancy disorder of yet unknown cause, missing means of treatment, and our fundamental understanding of the disease is still impaired. The purpose of this discovery study was to define candidate placenta tissue protein biomarker signatures to further decipher the molecular features of PE. Experimental design: We used recombinant antibody microarrays for multiplexed protein expression profiling of preeclamptic placenta tissue (n=25) versus normal placenta (n=11) targeting mainly immunoregulatory water-soluble proteins and membrane proteins. Furthermore, the three known subgroups of PE were profiled, including women with early onset preeclampsia and late onset preeclampsia, as well as women with PE and bilateral notching and intrauterine growth restrictions. Results: The data showed that the first generation of candidate PE-associated placenta tissue protein signatures were delineated, indicating that PE (receiver operating characteristics (ROC) AUC value of 0.83) and the subgroups thereof (ROC AUC values |
| Databáze: | OpenAIRE |
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