The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3
| Autor: | Keunsoo Kang, Eun Shik Choi, Daeyoup Lee, Youngseo Cheon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Chromosomal Proteins Non-Histone Science Centromere General Physics and Astronomy Cell Cycle Proteins macromolecular substances Biology General Biochemistry Genetics and Molecular Biology Chromatin remodeling Article Epigenesis Genetic 3-Isopropylmalate Dehydrogenase Histones 03 medical and health sciences Histone H3 Histone H1 Centromere Protein A Histone H2A Schizosaccharomyces Nucleosome Histone code lcsh:Science Genetics Multidisciplinary General Chemistry Chromatin Assembly and Disassembly Cell biology 030104 developmental biology Multiprotein Complexes lcsh:Q Schizosaccharomyces pombe Proteins |
| Zdroj: | Nature Communications, Vol 8, Iss 1, Pp 1-10 (2017) NATURE COMMUNICATIONS(8) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-017-00704-3 |
| Popis: | The centromere is the chromosomal locus at which the kinetochore is assembled to direct chromosome segregation. The histone H3 variant, centromere protein A (CENP-A), is known to epigenetically mark active centromeres, but the mechanism by which CENP-A propagates at the centromere, replacing histone H3, remains poorly understood. Using fission yeast, here we show that the Ino80 adenosine triphosphate (ATP)-dependent chromatin-remodeling complex, which removes histone H3-containing nucleosomes from associated chromatin, promotes CENP-ACnp1 chromatin assembly at the centromere in a redundant manner with another chromatin-remodeling factor Chd1Hrp1. CENP-ACnp1 chromatin actively recruits the Ino80 complex to centromeres to elicit eviction of histone H3-containing nucleosomes. Artificial targeting of Ino80 subunits to a non-centromeric DNA sequence placed in a native centromere enhances the spreading of CENP-ACnp1 chromatin into the non-centromeric DNA. Based on these results, we propose that CENP-ACnp1 chromatin employs the Ino80 complex to mediate the replacement of histone H3 with CENP-ACnp1, and thereby reinforces itself. The histone variant CENP-A marks active centromeres and replaces H3 at centromeres through a poorly understood mechanism. Here, the authors provide evidence that the chromatin remodeller Ino80 promotes CENP-A chromatin assembly at the centromere in fission yeast. |
| Databáze: | OpenAIRE |
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