Toward the Full Set of Human Mitochondrial Aminoacyl-tRNA Synthetases: Characterization of AspRS and TyrRS †
| Autor: | Aurélie Fender, Luc Bonnefond, Richard Giegé, Catherine Florentz, Marie Sissler, Joëlle Rudinger-Thirion |
|---|---|
| Přispěvatelé: | Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Mitochondrial DNA
Sequence analysis MESH: Mitochondria [SDV]Life Sciences [q-bio] Aspartate-tRNA Ligase Molecular Sequence Data MESH: RNA Transfer Asp MESH: Amino Acid Sequence Mitochondrion Biology MESH: Base Sequence MESH: Databases Nucleic Acid Biochemistry environment and public health MESH: Recombinant Proteins 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tyrosine-tRNA Ligase MESH: Tyrosine-tRNA Ligase MESH: RNA Transfer Tyr Humans MESH: Cloning Molecular Amino Acid Sequence MESH: Aspartate-tRNA Ligase Cloning Molecular Gene Peptide sequence 030304 developmental biology Genetics RNA Transfer Asp 0303 health sciences MESH: Humans MESH: Molecular Sequence Data Base Sequence Aminoacyl tRNA synthetase Computational Biology RNA Recombinant Proteins Mitochondria RNA Transfer Tyr MESH: Transfer RNA Aminoacylation chemistry Transfer RNA Transfer RNA Aminoacylation Databases Nucleic Acid 030217 neurology & neurosurgery MESH: Computational Biology |
| Zdroj: | Biochemistry Biochemistry, American Chemical Society, 2005, 44 (12), pp.4805-4816. ⟨10.1021/bi047527z⟩ |
| ISSN: | 0006-2960 1520-4995 |
| DOI: | 10.1021/bi047527z⟩ |
| Popis: | International audience; The human mitochondrion possesses a translational machinery devoted to the synthesis of 13 proteins. While the required tRNAs and rRNAs are produced by transcription of the mitochondrial genome, all other factors needed for protein synthesis are synthesized in the cytosol and imported. This is the case for aminoacyl-tRNA synthetases, the enzymes which esterify their cognate tRNA with the specific amino acid. The genes for the full set of cytosolic aaRSs are well defined, but only nine genes for mitochondrial synthetases are known. Here we describe the genes for human mitochondrial aspartyl- and tyrosyl-tRNA synthetases and the initial characterization of the enzymes. Both belong to the expected class of synthetases, have a dimeric organization, and aminoacylate Escherichia coli tRNAs as well as in vitro transcribed human mitochondrial tRNAs. Genes for the remaining missing synthetases were also found with the exception of glutaminyl-tRNA synthetase. Their sequence analysis confirms and further extends the view that, except for lysyl- and glycyl-tRNA synthetases, human mitochondrial and cytosolic enzymes are coded by two different sets of genes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|