Recombinant Modified Vaccinia Virus Ankara (MVA) Vaccines Efficiently Protect Cockatiels Against Parrot Bornavirus Infection and Proventricular Dilatation Disease

Autor:	Ralf Amann, Isabell Rall, Christiane Herden, Dennis Rubbenstroth, Sara Malberg
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0301 basic medicine Cockatiels animal structures 040301 veterinary sciences viruses Cockatoos Vaccinia virus Newcastle disease Article Virus proventricular dilatation disease (PDD) parrot bornavirus 4 (PaBV-4) 0403 veterinary science Viral Proteins 03 medical and health sciences chemistry.chemical_compound Parrots Proventricular dilatation disease Virology Animals Medicine modified vaccinia virus Ankara (MVA) parrot bornavirus 2 (PaBV-2) biology Bird Diseases business.industry Mononegavirales Infections Viral Vaccines 04 agricultural and veterinary sciences recombinant viral vector vaccines biology.organism_classification avian bornaviruses Vaccination 030104 developmental biology Infectious Diseases chemistry Immunization Bornaviridae RNA Viral Vaccinia business Viral load
Zdroj:	Viruses Volume 11 Issue 12
ISSN:	1999-4915
DOI:	10.3390/v11121130
Popis:	Parrot bornaviruses (PaBVs) are the causative agents of proventricular dilatation disease (PDD), a chronic and often fatal neurologic disorder in Psittaciformes. The disease is widely distributed in private parrot collections and threatens breeding populations of endangered species. Thus, immunoprophylaxis strategies are urgently needed. In previous studies we demonstrated a prime-boost vaccination regime using modified vaccinia virus Ankara (MVA) and Newcastle disease virus (NDV) constructs expressing the nucleoprotein and phosphoprotein of PaBV-4 (MVA/PaBV-4 and NDV/PaBV-4, respectively) to protect cockatiels (Nymphicus hollandicus) against experimental challenge infection. Here we investigated the protective effect provided by repeated immunization with either MVA/PaBV-4, NDV/PaBV-4 or Orf virus constructs (ORFV/PaBV-4) individually. While MVA/PaBV-4-vaccinated cockatiels were completely protected against subsequent PaBV-2 challenge infection and PDD-associated lesions, the course of the challenge infection in NDV/PaBV-4- or ORFV/PaBV-4-vaccinated birds did not differ from the unvaccinated control group. We further investigated the effect of vaccination on persistently PaBV-4-infected cockatiels. Remarkably, subsequent immunization with MVA/PaBV-4 and NDV/PaBV-4 neither induced obvious immunopathogenesis exacerbating the disease nor reduced viral loads in the infected birds. In summary, we demonstrated that vaccination with MVA/PaBV-4 alone is sufficient to efficiently prevent PaBV-2 challenge infection in cockatiels, providing a suitable vaccine candidate against avian bornavirus infection and bornavirus-induced PDD.
Databáze:	OpenAIRE
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