l-amino acid ligase: A promising alternative for the biosynthesis of l-dipeptides
| Autor: | Rui Li, Li-xiao Ning, Yu-fei Zhang, Yi-fan Wang, Xiao-huan Liu, Tao Wang, Xiang’e Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Bioengineering Protein Engineering 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Substrate Specificity Ligases Industrial Microbiology 03 medical and health sciences chemistry.chemical_compound Biosynthesis 010608 biotechnology Amino Acids chemistry.chemical_classification DNA ligase Bacteria Chemistry Dipeptides Protein engineering Combinatorial chemistry Amino acid 030104 developmental biology Biocatalysis Substrate specificity Biotechnology |
| Zdroj: | Enzyme and Microbial Technology. 136:109537 |
| ISSN: | 0141-0229 |
| Popis: | Given their special action mechanisms and structural simplicity, L-amino acid ligases (Lals) are considered to be desirable tools for the catalytic biosynthesis of dipeptides. Ywf E (BacD) was the first Lal identified and was shown to be involved in the biosynthesis of a potent antibacterial, bacilysin, since then, various novel Lals have been discovered. Each Lal has different substrate spectra and is capable of synthesizing different dipeptides. Owning to their great potentials for producing bioactive dipeptides of industrial importance, in this review, recent developments of Lals are discussed, including their structures, action mechanisms, applications and the advantages and disadvantages of different Lals. In addition, protein engineering of Lals to improve their substrate specificity and catalytic performance is also discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect