Functional and immunogenetic characterization of FcR-blocking antibody
| Autor: | Klara Mayer, Ágnes Padányi, Éva Gyódi, Gabriella Sármay, Petrányi G, Marianna Réti, M. Kassai |
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| Rok vydání: | 1990 |
| Předmět: |
Blood Platelets
Male Isoantigens Genotype Immunology chemical and pharmacologic phenomena Cell Separation Platelet Transfusion Receptors Fc Buffy coat Immunoglobulin E Antibodies Absorption HLA Antigens Renal Dialysis Blocking antibody Humans Immunology and Allergy Blood Transfusion Platelet Lymphocytes Whole blood Immunosuppression Therapy biology Graft Survival Kidney Transplantation Immunoglobulin Isotypes Transplantation Lymphocyte Transfusion biology.protein Female Immunization Antibody CD8 |
| Zdroj: | Immunology Letters. 26:131-137 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/0165-2478(90)90135-d |
| Popis: | The characteristics and functional importance of FcR-blocking antibodies and their production were investigated after immunization with whole blood, "buffy coat" and purified platelets. We studied the presence of FcR-blocking antibody in haemodialyzed, transfused patients waiting for kidney transplantation, and we found strong correlation between the blocking effect and better graft survival. We suggest that this blocking antibody does not attack FcR as primary target. Investigation of blocking activity of ten different immune sera on 50 healthy panel cells showed that target antigen has some polymorphic varieties. On basis of family studies it seems that the target antigen is not linked to HLA haplotype. The blocking effect of sera could be removed by absorption of CD8+ cells, B lymphocytes, platelets and granulocytes, but not with erythrocytes, monocytes, CD8- cells and NK cells. |
| Databáze: | OpenAIRE |
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