Safety and efficacy of global intracoronary administration of cardiosphere-derived cells or conditioned medium immediately after coronary reperfusion in rats
| Autor: | Maria Nana, John N. Nanas, Chris J. Kapelios, Ioannis Nanas, Styliani Vakrou, Michael J. Bonios, Emmeleia Nana-Leventaki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cardiac function curve
Male medicine.medical_specialty lcsh:Diseases of the circulatory (Cardiovascular) system Rat model Ischemia-reperfusion injury Myocardial Reperfusion Stem cells 030204 cardiovascular system & hematology Coronary reperfusion 03 medical and health sciences 0302 clinical medicine Internal medicine Intracoronary delivery medicine Conditioned medium Animals 030212 general & internal medicine Myocardial infarction business.industry Myocardium High cell medicine.disease Infarct size Rats Paracrinic function Disease Models Animal lcsh:RC666-701 Culture Media Conditioned Cardiology Histopathology Female Cardiology and Cardiovascular Medicine business |
| Zdroj: | Hellenic Journal of Cardiology, Vol 61, Iss 4, Pp 256-261 (2020) |
| ISSN: | 1109-9666 |
| Popis: | Objective Cardiosphere-derived cells (CDCs) have been shown to reduce infarct size after myocardial infarction (MI). In the present study we investigated the safety and efficacy of global intracoronary administration (GIA) of CDCs or CDC-conditioned medium (CM) immediately after reperfusion in a rat model of ischemia-reperfusion. Methods CDCs were grown from myocardial biopsies obtained from male Wistar Kyoto rats (WKY). Female WKY rats underwent MI for 45minutes, followed by reperfusion for 1hour. Infarcted rats were randomized to receive GIA of CDCs (CDC group), CM (CM group) or vehicle (control group) immediately after the onset of reperfusion. Cell retention was quantified by PCR for the male specific SRY gene; area at risk (AR) and no reflow area (NR) were measured by histopathology. Cardiac function was evaluated by echocardiography at 1 and 2 months post-MI. Results Cell retention at 1hour after GIA was 25.1% ±5.1. The myocardial AR and NR (measured at 1 hour post-reperfusion) were similar between groups [AR: 28.8% ±7.4 of LV mass in control vs 27.2% ±8 in CM vs 27% ±7 in CDCs group. NR: 7.0% ±3.3 in control vs 7.3% ±3.8 in CM vs 7.1% ±3.6 in CDCs]. One and 2 months post-MI, systolic function and LV volumes did not differ between control and CM groups. Conclusion Intracoronary administration of CDCs during the acute phase of MI, at the beginning of reperfusion, does not aggravate microvascular obstruction and results in high cell retention. Delivery of CM in the acute phase of MI did not confer long-term cardiac functional benefits. |
| Databáze: | OpenAIRE |
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