Interactions between odorants and glutathione transferases in the human olfactory cleft
| Autor: | Mathieu Schwartz, Loïc Briand, Franck Ménétrier, Philippe Faure, Evelyne Chavanne, Frédéric Lirussi, Marc Labrousse, Fabrice Neiers, Francis Canon, Bengt Mannervik, Jean-Marie Heydel |
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| Přispěvatelé: | Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Reims Champagne-Ardenne (URCA), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Stockholm University, French National Research Agency, French National Research Institute for Agriculture, Food and Environment and Swedish Research Council ( 2015-04222)., Oxford University Press, ANR-16-CE21-0004,OLFZYME,Olfaction et enzymes olfactives(2016) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Physiology Olfaction 03 medical and health sciences Behavioral Neuroscience GSTP1 chemistry.chemical_compound 0302 clinical medicine Olfactory Mucosa Physiology (medical) glutathione transferase medicine [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Humans human Receptor odorant chemistry.chemical_classification biology musculoskeletal neural and ocular physiology [SCCO.NEUR]Cognitive science/Neuroscience Cytochrome P450 Transporter Glutathione Sensory Systems 3. Good health 030104 developmental biology medicine.anatomical_structure Enzyme GSTA1 chemistry Biochemistry Odorants biology.protein Olfactory epithelium [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition 030217 neurology & neurosurgery psychological phenomena and processes olfaction |
| Zdroj: | Chemical Senses Chemical Senses, Oxford University Press (OUP), 2020, 45 (8), pp.645-654. ⟨10.1093/chemse/bjaa055⟩ |
| ISSN: | 0379-864X 1464-3553 |
| Popis: | Xenobiotic metabolizing enzymes and other proteins, including odorant-binding proteins located in the nasal epithelium and mucus, participate in a series of processes modulating the concentration of odorants in the environment of olfactory receptors (ORs) and finely impact odor perception. These enzymes and transporters are thought to participate in odorant degradation or transport. Odorant biotransformation results in 1) changes in the odorant quantity up to their clearance and the termination of signaling and 2) the formation of new odorant stimuli (metabolites). Enzymes, such as cytochrome P450 and glutathione transferases (GSTs), have been proposed to participate in odorant clearance in insects and mammals as odorant metabolizing enzymes. This study aims to explore the function of GSTs in human olfaction. Using immunohistochemical methods, GSTs were found to be localized in human tissues surrounding the olfactory epithelium. Then, the activity of 2 members of the GST family toward odorants was measured using heterologously expressed enzymes. The interactions/reactions with odorants were further characterized using a combination of enzymatic techniques. Furthermore, the structure of the complex between human GSTA1 and the glutathione conjugate of an odorant was determined by X-ray crystallography. Our results strongly suggest the role of human GSTs in the modulation of odorant availability to ORs in the peripheral olfactory process. |
| Databáze: | OpenAIRE |
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