Formaldehyde quantification using ampicillin is not selective
| Autor: | Tobias John, Raphael Reinbold, Akane Kawamura, Paolo Spingardi, Richard J. Hopkinson, Christopher J. Schofield, Amber L. Thompson |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell biology Quantification methods Metabolite Glyoxylate cycle Formaldehyde lcsh:Medicine Biochemistry 01 natural sciences Article Adduct 03 medical and health sciences chemistry.chemical_compound Ampicillin medicine lcsh:Science Multidisciplinary Chromatography 010405 organic chemistry lcsh:R Fluorescence 0104 chemical sciences 3. Good health 030104 developmental biology chemistry lcsh:Q Antibacterial activity medicine.drug |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-7 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Formaldehyde (HCHO) is a simple and highly reactive human metabolite but its biochemistry is poorly defined. A limiting factor in HCHO research is lack of validated quantification methods for HCHO relevant to biological samples. We describe spectroscopic studies on a reported fluorescence-based HCHO detection method involving its reaction with ampicillin. The results validate the structure and fluorescence properties of the HCHO-ampicillin reaction product. However, the same adduct is observed after reaction of ampicillin with glyoxylate. Related fluorophores were formed with other biologically relevant carbonyl compounds. Overall, our studies suggest the ampicillin method is not reliable for selective detection and quantification of HCHO in biological samples. |
| Databáze: | OpenAIRE |
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