Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains

Autor: Anderson Rodrigues dos Santos, Núbia Seyffert, Fernanda Alves Dorella, Anderson Miyoshi, Paula R. K. Falcão, Roberto Meyer, Robert J. Moore, Débora de Oliveira Lopes, Meritxell Zurita Turk, Maria Inês Tiraboschi Ferro, Ieso de Miranda Castro, Élida Mara Leite Rabelo, Juliana Franco Almeida, Thiago Luiz de Paula Castro, Diana Oliveira, Amjad Ali, Luis G.C. Pacheco, Daniela C. Bartholomeu, Vinicius A. C. Abreu, Glória Regina Franco, Anderson J. Dominitini, Sérgio Hermínio Brommonschenkel, Sílvia Regina Costa Dias, Newton Portilho Carneiro, Daniela de Melo Resende, Aryanne A. M. C. Rocha, Eva Trost, Marcília P. Costa, John A. McCulloch, Priscila Grynberg, Siomar de Castro Soares, Rommel Thiago Jucá Ramos, Santuza M. R. Teixeira, Elisângela Monteiro Coser, Ana Flávia D.V. Costa, Pablo M. R. O. Moraes, Jesus Aparecido Ferro, Sintia Almeida, Carlos Ueira Vieira, André Luiz Pedrosa, Artur Silva, Claudia Teixeira Guimarães, Sergio C. Oliveira, Jeronimo C. Ruiz, Anne Cybelle Pinto, Andreas Tauch, Luciano Vilela Paiva, Vivian D'Afonseca, Louise Cerdeira, Vasco Azevedo, José Miguel Ortega, Francisco Pereira Lobo, Guilherme Oliveira, Fabrício R. Santos, Jan Baumbach, Adhemar Zerlotini, Luiz Ricardo Goulart, Maria Paula Cruz Schneider, Luciana M. Oliveira
Přispěvatelé: JERÔNIMO C. RUIZ, Research Center René Rachou/Oswaldo Cruz Foundation, VÍVIAN D'AFONSECA, UFMG, ARTUR SILVA, UFPA, AMJAD ALI, UFMG, ANNE C. PINTO, UFMG, ANDERSON R. SANTOS, UFMG, ARYANNE A. M. C. ROCHA, UFMG, DÉBORA O. LOPES, UFSJ, FERNANDA A. DORELLA, UFMG, LUIS G. C. PACHECO, UFMG, UFBA, MARCÍLIA P. COSTA, UECE, MERITXELL Z. TURK, UFMG, NÚBIA SEYFFERT, UFMG, PABLO M. R. O. MORAES, UFMG, SIOMAR C. SOARES, UFMG, SINTIA S. ALMEIDA, UFMG, THIAGO L. P. CASTRO, UFMG, VINICIUS A. C. ABREU, UFMG, EVA TROST, University of Bielefeld, JAN BAUMBACH, Max-Planck-Institut fur Informatik, ANDREAS TAUCH, University of Bielefeld, MARIA PAULA C. SCHNEIDER, UFPA, JOHN MCCULLOCH, UFPA, LOUISE T. CERDEIRA, UFPA, ROMMEL T. J. RAMOS, UFPA, ADHEMAR ZERLOTINI, Research Center René Rachou/Oswaldo Cruz Foundation, ANDERSON DOMINITINI, Research Center René Rachou/Oswaldo Cruz Foundation, DANIELA M. RESENDE, Research Center René Rachou/Oswaldo Cruz Foundation, UFOP, ELISÂNGELA M. COSER, Research Center René Rachou/Oswaldo Cruz Foundation, LUCIANA M. OLIVEIRA, UFOP, ANDRÉ L. PEDROSA, UFOP, UFTM, CARLOS U. VIEIRA, UFU, CLÁUDIA TEIXEIRA GUIMARÃES, CNPMS, DANIELA C. BARTHOLOMEU, UFMG, DIANA M. OLIVEIRA, UECE, FABRÍCIO R. SANTOS, UFMG, ÉLIDA MARA RABELO, UFMG, FRANCISCO P. LOBO, UFMG, GLÓRIA R. FRANCO, UFMG, ANA FLÁVIA COSTA, UFMG, IESO M. CASTRO, UFOP, SÍLVIA REGINA COSTA DIAS, UFMG, JESUS A. FERRO, Unesp, JOSÉ MIGUEL ORTEGA, UFMG, LUCIANO V. PAIVA, UFV, LUIZ R. GOULART, UFU, JULIANA FRANCO ALMEIDA, UFU, MARIA INÊS T. FERRO, Unesp, NEWTON PORTILHO CARNEIRO, CNPMS, PAULA REGINA KUSER FALCÃO, CNPTIA, PRISCILA GRYNBERG, UFMG, SANTUZA M. R. TEIXEIRA, UFMG, SÉRGIO BROMMONSCHENKEL, UFV, SÉRGIO C. OLIVEIRA, UFMG, ROBERTO MEYER, UFBA, ROBERT J. MOORE, CSIRO Livestock Industries, ANDERSON MIYOSHI, UFMG, GUILHERME C. OLIVEIRA, Oswaldo Cruz Foundation, VASCO AZEVEDO, UFMG., CLAUDIA TEIXEIRA GUIMARAES, CNPMS, NEWTON PORTILHO CARNEIRO, CNPMS., Fundação Oswaldo Cruz, Universidade Federal de Minas Gerais (UFMG), Fed Univ Para, Universidade Federal de São João del-Rei (UFSJ), Univ Estadual Ceara, Univ Bielefeld, Max Planck Inst Informat, Universidade Federal de Ouro Preto (UFOP), Universidade Federal do Triângulo Mineiro (UFTM), Universidade Federal de Uberlândia (UFU), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Universidade Estadual Paulista (Unesp), Universidade Federal de Lavras (UFLA), Universidade Federal de Viçosa (UFV), Universidade Federal da Bahia (UFBA), CSIRO Livestock Ind
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
PLoS ONE, Vol 6, Iss 4, p e18551 (2011)
PLoS ONE
Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Popis: Made available in DSpace on 2013-09-27T14:53:33Z (GMT). No. of bitstreams: 1 WOS000289620100009.pdf: 394055 bytes, checksum: 9a23eb5b1d44e130bf137337fc894796 (MD5) Previous issue date: 2011-04-18 Made available in DSpace on 2013-09-30T18:01:03Z (GMT). No. of bitstreams: 1 WOS000289620100009.pdf: 394055 bytes, checksum: 9a23eb5b1d44e130bf137337fc894796 (MD5) Previous issue date: 2011-04-18 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:17:54Z No. of bitstreams: 1 WOS000289620100009.pdf: 394055 bytes, checksum: 9a23eb5b1d44e130bf137337fc894796 (MD5) Made available in DSpace on 2014-05-20T13:17:54Z (GMT). No. of bitstreams: 1 WOS000289620100009.pdf: 394055 bytes, checksum: 9a23eb5b1d44e130bf137337fc894796 (MD5) Previous issue date: 2011-04-18 Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) NIH (National Institutes of Health) - Fogarty Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Background: Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity.Methodology and Findings: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal transfer.Conclusions: These particular genome characteristics of C. pseudotuberculosis, as well as its acquired virulence factors in pathogenicity islands, provide evidence of its lifestyle and of the pathogenicity pathways used by this pathogen in the infection process. All genomes cited in this study are available in the NCBI Genbank database (http://www.ncbi.nlm.nih.gov/genbank/) under accession numbers CP001809 and CP001829. Fundação Oswaldo Cruz, Res Ctr Rene Rachou, Ctr Excellence Bioinformat, Natl Inst Sci & Technol, Belo Horizonte, MG, Brazil Universidade Federal de Minas Gerais (UFMG), Dept Gen Biol, Belo Horizonte, MG, Brazil Fed Univ Para, Dept Genet, BR-66059 Belem, Para, Brazil Univ Fed Sao Joao del Rei, Hlth Sci Ctr, Divinopilis, MG, Brazil Univ Estadual Ceara, Dept Vet Med, Fortaleza, Ceara, Brazil Univ Bielefeld, Dept Genet, CeBiTech, Bielefeld, Nordrhein Westf, Germany Max Planck Inst Informat, Dept Comp Sci, Saarbrucken, Saarlan, Germany Univ Fed Ouro Preto, Dept Pharmaceut Sci, Ouro Preto, MG, Brazil Univ Fed Ouro Preto, Dept Phis, Ouro Preto, MG, Brazil Univ Fed Triangulo Mineiro, Dept Biol Sci, Uberaba, MG, Brazil Universidade Federal de Uberlândia (UFU), Dept Biochem & Genet, BR-38400 Uberlandia, MG, Brazil Brazilian Agr Res Corp Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Sete Lagoas, MG, Brazil Universidade Federal de Minas Gerais (UFMG), Dept Biochem & Immunol, Belo Horizonte, MG, Brazil Universidade Federal de Minas Gerais (UFMG), Dept Parasitol, Belo Horizonte, MG, Brazil Univ Fed Ouro Preto, Dept Pharm, Ouro Preto, MG, Brazil State Univ São Paulo, Dept Technol, Jaboticabal, SP, Brazil Universidade Federal de Lavras (UFLA), Dept Chem, Lavras, MG, Brazil Brazilian Agr Res Corp Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Campinas, SP, Brazil Universidade Federal de Viçosa (UFV), Dept Plant Pathol, Vicosa, MG, Brazil Universidade Federal da Bahia (UFBA), Dept Biointeract Sci, Salvador, BA, Brazil CSIRO Livestock Ind, Clayton, Vic, Australia State Univ São Paulo, Dept Technol, Jaboticabal, SP, Brazil FAPEMIG: CBB-1181/0 FAPEMIG: REDE-186/08 NIH - Fogarty: TW007012
Databáze: OpenAIRE
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