microRNA-155 Modulates Hepatic Stellate Cell Proliferation, Apoptosis, and Cell Cycle Progression in Rats With Alcoholic Hepatitis via the MAPK Signaling Pathway Through Targeting SOCS1
| Autor: | Shulan Liu, Ping Han, Xiaocui Yao, Wei Gao, Chunhai Gao, Dengtao Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
0301 basic medicine
Hepatic stellate cell proliferation MAPK/ERK pathway microRNA-155 alcoholic hepatitis RM1-950 03 medical and health sciences 0302 clinical medicine Pharmacology (medical) proliferation and apoptosis 3 proliferation and apoptosis Original Research mitogen activated protein kinase signaling pathway hepatic stellate cell Pharmacology Chemistry Suppressor of cytokine signaling 1 Liver cell lcsh:RM1-950 Retraction 030104 developmental biology lcsh:Therapeutics. Pharmacology suppressor of cytokine signaling 1 Apoptosis 030220 oncology & carcinogenesis Cancer research Hepatic stellate cell Therapeutics. Pharmacology Signal transduction TBIL mitogen-activated protein kinase signaling pathway |
| Zdroj: | Frontiers in Pharmacology Frontiers in Pharmacology, Vol 11 (2020) Frontiers in Pharmacology, Vol 12 (2022) |
| ISSN: | 1663-9812 |
| Popis: | The aim of this study was to investigate the regulatory function of the non-coding microRNA-155 (miR-155) and suppressor of cytokine signaling 1 (SOCS1) in alcoholic hepatitis (AH) and its potential mechanism associated with the mitogen-activated protein kinase (MAPK) signaling pathway. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), total bilirubin (TBIL), malondialdehyde (MDA), and superoxide dismutase (SOD) were measured in a rat model of AH. The biological prediction website microRNA.org and dual-luciferase reporter gene assay were used to identify whether SOCS1 was a direct target of miR-155, and the effects of miR-155 and SOCS1 on the viability, cycle progression, and apoptosis of hepatic stellate cells were assessed using RT-qPCR, Western blot assay, MTT assay, Annexin V/PI double staining, and PI single staining. The levels of ALT, AST, MDA, and TBIL and the liver cell morphology were all prominently changed in AH model rats. miR-155 suppressed SOCS1 by specifically binding to SOCS1-3’-UTR to activate the MAPK signaling pathway. SOCS1 had low expression while miR-155 was highly expressed in AH rats. miR-155 promoted hepatic stellate cell viability and cycle progression and reduced cell apoptosis by silencing SOCS1. Together, we find that silenced miR-155 could upregulate SOCS1 and inactivate the MAPK signaling pathway, thereby inhibiting the proliferation of alcoholic hepatic stellate cells and promoting cell apoptosis. |
| Databáze: | OpenAIRE |
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