Detection and discrimination of cereal and leguminous species in chestnut flour by duplex PCR
| Autor: | Cyril Buissonade, Rémi Alary, Philippe Joudrier, Marie-Françoise Gautier |
|---|---|
| Přispěvatelé: | Développement et amélioration des plantes (UMR DAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
0106 biological sciences
FEVE Biology 01 natural sciences Biochemistry Industrial and Manufacturing Engineering law.invention chemistry.chemical_compound 0404 agricultural biotechnology law Botany [SDV.IDA]Life Sciences [q-bio]/Food engineering Food science SEIGLE Common wheat Gene Polymerase chain reaction Analysis method CEREALS 2. Zero hunger FOOD LABELING CHESTNUT food and beverages 04 agricultural and veterinary sciences General Chemistry DNA AVOINE HARICOT 040401 food science Thionin Food labeling Duplex pcr LEGUMES PCR chemistry POIS CHICHE Control methods 010606 plant biology & botany Food Science Biotechnology |
| Zdroj: | European Food Research and Technology European Food Research and Technology, Springer Verlag (Germany), 2007, 225 (3-4), pp.427-434. ⟨10.1007/s00217-006-0434-6⟩ |
| ISSN: | 1438-2377 1438-2385 |
| Popis: | Equipe DGB, ex-UMR PIA; International audience; Chestnut and chestnut-derived products are quite expensive and thus a possible target for fraudulent labeling. To obtain a French Label of Origin, chestnut producers need to certify the purity of their products. Chestnut-derived products are consumed by people who may suffer from celiac disease or are allergic to cereals. For these reasons, we developed a qualitative PCR-based method to detect cereal and leguminous species in chestnut flour. The presence of common wheat and barley was determined by amplifying part of the puroindoline-a gene, the presence of rye by amplifying part of the secaloindoline-a gene, the presence of durum wheat, rice, maize and chickpea by amplifying part of lipid transfer protein genes, the presence of oat by amplifying part of a thionin gene, the presence of kidney bean by amplifying part of a late embryogenesis- abundant protein, the presence of soybean by amplifying part of a lectin gene and the presence of fava bean by amplifying part of a nodulin gene. A chestnut thaumatine gene was used as control. PCR conditions were optimized to detect 1% of adulteration. Duplex PCR and specially designed sets of primers that allow amplification of several related species limited the number and cost of analyses required. Interpretation of the results is facilitated by using a decision tree |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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