Active Control of Dye Release for Neuronal Tracing Using PEDOT-PSS Coated Electrodes
Autor: | Maria Asplund, Antje Kilias, Ulrich Egert, Patrick Ruther, Stefanie Heizmann |
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Rok vydání: | 2016 |
Předmět: |
Materials science
Biomedical Engineering Nanotechnology 02 engineering and technology Thiophenes engineering.material Cell Line 03 medical and health sciences 0302 clinical medicine Coating PEDOT:PSS Coated Materials Biocompatible Internal Medicine Electric Impedance Humans Coloring Agents Leakage (electronics) Conductive polymer Neurons General Neuroscience Bilayer Rehabilitation Electrochemical Techniques 021001 nanoscience & nanotechnology Culture Media Electrodes Implanted Ion Exchange Microelectrode Electrode engineering Polystyrenes 0210 nano-technology Layer (electronics) Microelectrodes 030217 neurology & neurosurgery Biomedical engineering |
Zdroj: | IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society. 26(2) |
ISSN: | 1558-0210 |
Popis: | Penetrating neural probes comprising arrays of microelectrodes are commonly used to monitor local field potentials and multi-unit activity in animal brain over time frames of weeks. To correlate these recorded signals to specific tissue areas, histological analysis is performed after the experimental endpoint. Even if the lesion of the penetrating probe shaft can be observed, a precise reconstruction of the exact electrode positions is still challenging. To overcome these experimental difficulties, we developed a new concept, whereupon recording electrodes are coated with a poly (3, 4-ethylenedioxythiophene/ polystyrenesulfonate) (PEDOT/PSS)-based film. The conducting polymer acts as dye reservoir over several weeks and afterwards provides controlled delivery of neurotracers. This paper presents a recording electrode based on a PEDOT/PSS bilayer optimized for dye delivery and with reduced impedance. Controlled exchange of neurotracer dye is successfully demonstrated in vitro using spectrofluorometry and in neuroblastoma cell cultures. A second PEDOT/PSS capping layer on top of the dye reservoir lowers the passive leakage of dye by a factor of 6.4 and prevents a direct contact of the dye filled layer with the cells. Stability tests over four weeks demonstrate the electrochemical stability of the PEDOT coating, as well as retained functionality of the dye delivery system. |
Databáze: | OpenAIRE |
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