Can paraoxonase 1 polymorphisms (L55 M and Q192 R) protect children with type 1 diabetes against lipid abnormalities?
| Autor: | Ilhem Hellara, Mohamed Neji Guediche, S. Chouchane, Sonia Triki, Ali Bouslama, Mohamed Fadhel Najjar, Ons Fekih, Fadoua Neffati, Jihen Rejeb, Asma Ommezzine |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty Tunisia Adolescent Genotype Apolipoprotein B Endocrinology Diabetes and Metabolism Lipid Metabolism Disorders Young Adult chemistry.chemical_compound Gene Frequency Internal medicine Internal Medicine medicine Humans Child Genetics Polymorphism Genetic Nutrition and Dietetics biology medicine.diagnostic_test Aryldialkylphosphatase business.industry Cholesterol Haplotype Paraoxonase nutritional and metabolic diseases PON1 Apolipoproteins Diabetes Mellitus Type 1 Spectrometry Fluorescence Endocrinology Haplotypes chemistry biology.protein Female lipids (amino acids peptides and proteins) Apolipoprotein A1 Cardiology and Cardiovascular Medicine business Lipid profile Lipoprotein |
| Zdroj: | Journal of Clinical Lipidology. 8:249-255 |
| ISSN: | 1933-2874 |
| Popis: | Background Only a few studies have focused on the possible modulatory role of paraoxonase 1 (PON1) polymorphisms in lipid profiles, especially in children and in adolescents with type 1 diabetes (T1D). Objective We propose to study the association between PON1 polymorphisms (PON1-55 and PON1-192) and a lipid profile in a young Tunisian population with T1D. Methods The study compared 122 children and adolescents with T1D with 97 controls. Genomic DNA was collected from 116 patients and 91 controls. Lipid parameters were determined by automated methods. PON1 activity was measured by a spectrophotometric method and genotyping of the PON1 gene was assessed by multiplex polymerase chain reaction followed by restriction fragment-length polymorphism. Results A significant increase in total cholesterol, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol (LDL-C), apolipoprotein B (ApoB), and lipoprotein (a) (Lp(a)) and a significant decrease in apolipoprotein A1 (ApoA1), ApoA1/ApoB ratio, and PON1 activity/HDL-C ratio were observed in children with T1D compared with controls. In the LLQR haplotype, the group with diabetes showed significantly higher values of total cholesterol, LDL-C, apoB, Lp(a), and apoA1/apoB ratio compared with the control group. Those with diabetes with the LLQQ haplotype showed a significant decrease in LDL-C and Lp(a) compared with controls ( P Conclusion PON1 polymorphisms (PON1-55 and PON1-192) seem to be involved in the altering the lipid profile in T1D. The LLQR haplotype provided an atherogenic lipid profile in children with T1D compared with controls. LLQQ haplotype seemed to have a protective effect against the increase in LDL-C and Lp(a) that are heavily involved in the development of cardiovascular diseases. |
| Databáze: | OpenAIRE |
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