Immunoglobulin Heavy- And Light-chain Repertoire in Splenic Marginal Zone Lymphoma
| Autor: | Aikaterini Parasi, Theodora Papadaki, Chrysoula Belessi, Niki Stavroyianni, Stavroula Afendaki, Vassiliki Douka, Evangelia Kalagiakou, Kostas Stamatopoulos, Achilles Anagnostopoulos, Nikolaos Laoutaris, Dimitra Anagnostou, Athanasios Fassas, Riad Saloum |
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| Rok vydání: | 2004 |
| Předmět: |
Lymphoma
Gene Rearrangement B-Lymphocyte Heavy Chain Biology Immunoglobulin light chain Germline Immunoglobulin kappa-Chains Germline mutation Genotype Immunogenetics Genetics medicine Gene Rearrangement B-Lymphocyte Light Chain Humans Splenic marginal zone lymphoma Molecular Biology Gene Genetics (clinical) B cell Splenic Neoplasms Articles medicine.disease Complementarity Determining Regions medicine.anatomical_structure Molecular Medicine Immunoglobulin Light Chains Somatic Hypermutation Immunoglobulin Immunoglobulin Heavy Chains IGHV@ |
| Zdroj: | Molecular Medicine. 10:89-95 |
| ISSN: | 1528-3658 1076-1551 |
| DOI: | 10.2119/2005-00001.stamatopoulos |
| Popis: | The considerable heterogeneity in morphology, immunophenotype, genotype, and clinical behavior of splenic marginal zone lymphoma (SMZL) hinders firm conclusions on the origin and differentiation stage of the neoplastic cells. Immunoglobulin (IG) gene usage and somatic mutation patterns were studied in a series of 43 SMZL cases. Clonal IGHV-D-J rearrangements were amplified in 42/43 cases (4 cases carried double rearrangements). Among IGHV-D-J rearrangements, IGHV3 and IGHV4 subgroup genes were used with the highest frequency. Nineteen IGHV genes were unmutated (>98% homology to the closest germline IGHV gene), whereas 27/46 were mutated. Clonal IGKV-J and IGLV-J gene rearrangements were amplified in 36/43 cases, including 31 IGKV-J (8/31 in lambda light-chain expressing cases) and 12 IGLV-J rearrangements; 9/31 IGKV and 6/12 IGLV sequences were mutated. IGKV-J and IGLV-J rearrangements used 14 IGKV and 9 IGLV different germline genes. Significant evidence for positive selection by classical T-dependent antigen was found in only 5/27 IGHV and 6/15 IGKV+IGLV mutated genes. These results provide evidence for the diverse B-cell subpopulations residing in the SMZ, which could represent physiologic equivalents of distinct SMZL subtypes. Furthermore, they indicate that in SMZL, as in other B cell malignancies, a complementarity imprint of antigen selection might be witnessed either by IGHV, IGKV, or IGLV rearranged sequences. |
| Databáze: | OpenAIRE |
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