Borrelia burgdorferi BBA52 is a potential target for transmission blocking Lyme disease vaccine
| Autor: | Manish Kumar, Simarjot Kaur, Toru Kariu, Ioannis Bossis, Xiuli Yang, John F. Anderson, Utpal Pal |
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| Rok vydání: | 2011 |
| Předmět: |
Active immunization
Article Microbiology Mice Immune system Lyme disease Antigen medicine Animals Borrelia burgdorferi Antigens Bacterial Lyme Disease Mice Inbred C3H Ixodes General Veterinary General Immunology and Microbiology biology Immunization Passive Public Health Environmental and Occupational Health Lyme Disease Vaccines bacterial infections and mycoses biology.organism_classification medicine.disease Antibodies Bacterial Virology Infectious Diseases Immunization biology.protein Molecular Medicine Antibody Bacterial Outer Membrane Proteins |
| Zdroj: | Vaccine. 29:9012-9019 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2011.09.035 |
| Popis: | The surface-exposed antigens of Borrelia burgdorferi represent important targets for induction of protective host immune responses. BBA52 is preferentially expressed by B. burgdorferi in the feeding tick, and a targeted deletion of bba52 interferes with vector–host transitions in vivo. In this study, we demonstrate that BBA52 is an outer membrane surface-exposed protein and that disulfide bridges take part in the homo-oligomeric assembly of native protein. BBA52 antibodies lack detectable borreliacidal activities in vitro. However, active immunization studies demonstrated that BBA52 vaccinated mice were significantly less susceptible to subsequent tick-borne challenge infection. Similarly, passive transfer of BBA52 antibodies in ticks completely blocked B. burgdorferi transmission from feeding ticks to naive mice. Taken together, these studies highlight the role of BBA52 in spirochete dissemination from ticks to mice and demonstrate the potential of BBA52 antibody-mediated strategy to complement the ongoing efforts to develop vaccines for blocking the transmission of B. burgdorferi . |
| Databáze: | OpenAIRE |
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