Glutathione (GSH) reduces the open probability of mechanosensitive channels in Escherichia coli protoplasts
| Autor: | Piotr Koprowski, Andrzej Kubalski |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
inorganic chemicals
Patch-Clamp Techniques Physiology Clinical Biochemistry medicine.disease_cause Ion Channels Dithiothreitol chemistry.chemical_compound Physiology (medical) Escherichia coli Pressure medicine Patch clamp Ion channel Protoplasts Cell Membrane Electric Conductivity Glutathione Biomechanical Phenomena Membrane chemistry Biochemistry Cytoplasm Biophysics Mechanosensitive channels Oxidation-Reduction |
| Zdroj: | Pfl�gers Archiv European Journal of Physiology. 438:361-364 |
| ISSN: | 1432-2013 0031-6768 |
| DOI: | 10.1007/s004240050921 |
| Popis: | The effects of glutathione (reduced GSH, and oxidized GSSG) on mechanosensitive (MS) ion channels from Escherichia coli protoplasts were investigated using excised, inside-out membrane patches. Our studies demonstrate here that 5 mM GSH irreversibly reduces the activities of the 560-pS MS channel by approximately 70-75% whereas 5 mM GSSG did not alter the MS channel currents. In addition, millimolar concentrations of dithiothreitol had similar although weaker effects to GSH. The physiological concentration of GSH in E. coli cytoplasm ranges from 3.5 mM to 6.6 mM, which may indicate that under normal conditions these MS channels open less due to membrane stretch. |
| Databáze: | OpenAIRE |
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