MiR-15b regulates cell differentiation and survival by targeting CCNE1 in APL cell lines
Autor: | Lu Liu, Beizhong Liu, Juanjuan Yao, Zhen Yuan, Shifei Yao, Yi Zhao, Junmei Liu, Pengqiang Zhong, Min Chen, Liang Zhong, Lianwen Li, Dongdong Liu |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Acute promyelocytic leukemia Myeloid Cellular differentiation Cell HL-60 Cells Biology 03 medical and health sciences 0302 clinical medicine Leukemia Promyelocytic Acute Cyclin E medicine Humans Cell Proliferation Oncogene Proteins Cell growth Cell Differentiation Cell Biology Cell cycle medicine.disease Cell biology MicroRNAs 030104 developmental biology medicine.anatomical_structure Cell culture Apoptosis 030220 oncology & carcinogenesis |
Zdroj: | Cellular Signalling. 60:57-64 |
ISSN: | 0898-6568 |
Popis: | MicroRNAs have been shown to be involved in various cell processes, including proliferation, apoptosis and differentiation. However, little is known about their function in granulopoiesis. In the present study, overexpression and knockdown experiments revealed that miR-15b was required to block the proliferation of NB4 and HL60 cells and induce them differentiated to granulocyte lineage. Moreover, we identified CCNE1 as a direct target of miR-15b, and demonstrated that CCNE1 was involved in cell differentiation and proliferation in acute promyelocytic leukemia cells. In addition, we demonstrated a novel pathway in which miR-15b regulated cells arrested in the G0/G1 phase and promoted terminal differentiation of cells by targeting CCNE1, which could modulate the cell cycle effort pRb in APL cells. These events blocked cell proliferation and promoted granulocyte differentiation. In conclusion, our data highlighted, for the first time, the important role of miR-15b in myeloid differentiation and suggested the potential role of miR-15b in cancer therapy. |
Databáze: | OpenAIRE |
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