Biomarkers of Broccoli Consumption: Implications for Glutathione Metabolism and Liver Health
| Autor: | Xiaoji Liu, Michael J. Miller, Elizabeth H. Jeffery, Yanling Wang, Zeynep Madak-Erdogan, Alicia Arredondo Eve |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty NF-E2-Related Factor 2 Metabolite Population Brassica lcsh:TX341-641 Gut flora liver Article 03 medical and health sciences Liver disease chemistry.chemical_compound Eating Metabolomics Metabolic Diseases Internal medicine medicine NAD(P)H Dehydrogenase (Quinone) Animals education Fatty acid synthesis broccoli education.field_of_study 030109 nutrition & dietetics Nutrition and Dietetics biology Fatty Acids food and beverages biology.organism_classification medicine.disease Glutathione Rats Inbred F344 Gastrointestinal Microbiome Glutamine 030104 developmental biology Endocrinology chemistry Metabolome Animal Nutritional Physiological Phenomena lcsh:Nutrition. Foods and food supply metabolic circulating markers Food Science |
| Zdroj: | Nutrients, Vol 12, Iss 2514, p 2514 (2020) Nutrients Volume 12 Issue 9 |
| ISSN: | 2072-6643 |
| Popis: | Diet and lifestyle choices contribute to obesity and liver disease. Broccoli, a brassica vegetable, may mitigate negative effects of both diet and lifestyle. Currently, there are no clinically relevant, established molecular biomarkers that reflect variability in human absorption of brassica bioactives, which may be the cause of variability/inconsistencies in health benefits in the human population. Here, we focused on the plasma metabolite profile and composition of the gut microbiome in rats, a relatively homogenous population in terms of gut microbiota, genetics, sex and diet, to determine if changes in the plasma metabolite profiles caused by dietary broccoli relate to molecular changes in liver. Our aim was to identify plasma indicators that reflect how liver health is impacted by dietary broccoli. Rats were fed a 10% broccoli diet for 14 days. We examined the plasma metabolite composition by metabolomics analysis using GC&ndash MS and gut microbiota using 16S sequencing after 0, 1, 2, 4, 7, 14 days of broccoli feeding. We identified 25 plasma metabolites that changed with broccoli consumption, including metabolites associated with hepatic glutathione synthesis, and with de novo fatty acid synthesis. Glutamine, stearic acid, and S-methyl-L-cysteine (SMC) relative abundance changes correlated with changes in gut bacteria previously implicated in metabolic disease and with validated increases in expression of hepatic NAD(P)H dehydrogenase [quinone] 1 (NQO1) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2), associated with elevated hepatic glutathione synthesis. Circulating biomarkers following broccoli consumption reflect gut&ndash liver axis health. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|