A Novel Approach to Maintain Gut Mucosal Integrity Using an Oral Enzyme Supplement
| Autor: | Richard A. Hodin, José Luis Millán, Tyler J. Tantillo, Qingsong Tao, Kanakaraju Kaliannan, Mohammad Hadi Gharedaghi, Gwendolyn M. van der Wilden, Sulaiman R. Hamarneh, Tanit Phupitakphol, Sara A. Morrison, Sarah S. Gul, Sonoko Narisawa, Peter J. Fagenholz, Konstantinos P. Economopoulos, Mussa M. Rafat Mohamed, Madhu S. Malo |
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| Rok vydání: | 2014 |
| Předmět: |
Critical Illness
Administration Oral Ileum Inflammation digestive system Enteral administration Permeability Article Sepsis Mice Enteral Nutrition Intestinal mucosa Animals Humans Medicine Intestinal Mucosa Tight Junction Proteins business.industry digestive oral and skin physiology Alkaline Phosphatase medicine.disease Systemic Inflammatory Response Syndrome Up-Regulation Systemic inflammatory response syndrome Jejunum medicine.anatomical_structure Parenteral nutrition Starvation Dietary Supplements Immunology Alkaline phosphatase Surgery medicine.symptom business |
| Zdroj: | Annals of Surgery. 260:706-715 |
| ISSN: | 0003-4932 |
| DOI: | 10.1097/sla.0000000000000916 |
| Popis: | To determine the role of intestinal alkaline phosphatase (IAP) in enteral starvation-induced gut barrier dysfunction and to study its therapeutic effect as a supplement to prevent gut-derived sepsis.Critically ill patients are at increased risk for systemic sepsis and, in some cases, multiorgan failure leading to death. Years ago, the gut was identified as a major source for this systemic sepsis syndrome. Previously, we have shown that IAP detoxifies bacterial toxins, prevents endotoxemia, and preserves intestinal microbiotal homeostasis.WT and IAP-KO mice were used to examine gut barrier function and tight junction protein levels during 48-hour starvation and fed states. Human ileal fluid samples were collected from 20 patients postileostomy and IAP levels were compared between fasted and fed states. To study the effect of IAP supplementation on starvation-induced gut barrier dysfunction, WT mice were fasted for 48 hours +/- IAP supplementation in the drinking water.The loss of IAP expression is associated with decreased expression of intestinal junctional proteins and impaired barrier function. For the first time, we demonstrate that IAP expression is also decreased in humans who are deprived of enteral feeding. Finally, our data demonstrate that IAP supplementation reverses the gut barrier dysfunction and tight junction protein losses due to a lack of enteral feeding.IAP is a major regulator of gut mucosal permeability and is able to ameliorate starvation-induced gut barrier dysfunction. Enteral IAP supplementation may represent a novel approach to maintain bowel integrity in critically ill patients. |
| Databáze: | OpenAIRE |
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