Inhibitory effects of curcumin against cytotoxicity of Porphyromonas gingivalis outer membrane vesicles
Autor: | Shinichi Sekine, Shusuke Izui, Hiroki Takeuchi, Hiroki Murai, Atsuo Amano |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Curcumin Gingiva Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cytotoxic T cell Humans Cytotoxicity General Dentistry Porphyromonas gingivalis biology Chemistry Cell migration Epithelial Cells 030206 dentistry Cell Biology General Medicine biology.organism_classification Molecular biology 030104 developmental biology Otorhinolaryngology Cytokines Bacterial outer membrane Intracellular |
Zdroj: | Archives of oral biology. 124 |
ISSN: | 1879-1506 |
Popis: | Objective The purpose of this study was to examine whether curcumin, a turmeric root extract, protects human gingival epithelial (HGE) cells from the cytotoxic effects ofPorphyromonas gingivalis outer membrane vesicles (OMVs). Design OMVs were prepared fromP. gingivalis OMZ314 and used to stimulate human gingival epithelial (HGE) cells. The effects of curcumin on cellular expression of inflammatory cytokines were evaluated using real-time reverse transcription-polymerase chain reaction assays, while those on cellular migration were examined with a scratch wound assay. Furthermore, HGE cells were incubated with OMVs in the presence or absence of curcumin, then intracellular invasion by OMVs was observed with confocal laser scanning microscopy. Also, the effects of curcumin on cellular apoptotic death was examined. Results Gene expressions of IL-6, IL-1β, and TNF-α in HGE cells stimulated with OMVs were significantly suppressed by curcumin in a dose-dependent manner, with suppressed protein production also noted. Moreover, curcumin inhibited the cytotoxic effects of OMVs on cellular migration. Finally, curcumin inhibited OMV adherence to and entry of cells, as well as cellular apoptotic death in a dose-dependent manner. Conclusions Curcumin showed marked inhibitory effects against the cytotoxic actions of P. gingivalis OMVs, indicating possible potency for preventing periodontal disease. |
Databáze: | OpenAIRE |
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