Toxic and genotoxic effects of the 2,4-dichlorophenoxyacetic acid (2,4-D)-based herbicide on the Neotropical fish Cnesterodon decemmaculatus

Autor: Marcelo L. Larramendy, Sonia Soloneski, C. Ruiz de Arcaute
Rok vydání: 2015
Předmět:
0301 basic medicine
2
4-Dichlorophenoxyacetic acid
Erythrocytes
DNA damage
Otras Ciencias Biológicas
Scge Assay
Health
Toxicology and Mutagenesis
Sublethal Effects
Micronuclei
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Median lethal dose
Ciencias Biológicas
purl.org/becyt/ford/1 [https]
Toxicology
Lethal Dose 50
03 medical and health sciences
chemistry.chemical_compound
Cyprinodontiformes
medicine
Animals
purl.org/becyt/ford/1.6 [https]
Behavioral Changes
Micronuclei
Chromosome-Defective
Swimming
0105 earth and related environmental sciences
Poeciliidae
Lethality
biology
Herbicides
Mutagenicity Tests
Public Health
Environmental and Occupational Health
General Medicine
biology.organism_classification
Pollution
Molecular biology
Acute toxicity
Comet assay
030104 developmental biology
chemistry
Micronucleus test
Comet Assay
2
4-Dichlorophenoxyacetic Acid
Agrochemicals
CIENCIAS NATURALES Y EXACTAS
Genotoxicity
Water Pollutants
Chemical
DNA Damage
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 1090-2414
Popis: Acute toxicity and genotoxicity of the 54.8% 2,4-D-based commercial herbicide DMA® were assayed on Cnesterodon decemmaculatus (Pisces, Poeciliidae). Whereas lethal effect was used as the end point for mortality, frequency of micronuclei (MNs), other nuclear abnormalities and primary DNA damage evaluated by the single cell gel electrophoresis (SCGE) assay were employed as end points for genotoxicity. Mortality studies demonstrated an LC50 96 h value of 1008 mg/L (range, 929-1070) of 2,4-D. Behavioral changes, e.g., gathering at the bottom of the aquarium, slowness in motion, slow reaction and abnormal swimming were observed. Exposure to 2,4-D within the 252-756 mg/L range increased the frequency of MNs in fish exposed for both 48 and 96 h. Whereas blebbed nuclei were induced in treatments lasting for 48 and 96 h, notched nuclei were only induced in fish exposed for 96 h. Regardless of both concentration and exposure time, 2,4-D did not induce lobed nuclei and binucleated erythrocytes. In addition, we found that exposure to 2,4-D within the 252-756 mg/L range increased the genetic damage index in treatments lasting for either 48 and 96 h. The results represent the first experimental evidence of the lethal and several sublethal effects, including behavioral alterations and two genotoxic properties namely the induction of MNs and primary DNA strand breaks, exerted by 2,4-D on an endemic organism as C. decemmaculatus. Fil: Ruiz de Arcaute, Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina Fil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina Fil: Larramendy, Marcelo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Databáze: OpenAIRE
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