Development of antibodies to and PCR detection of Ehrlichia spp. in dogs following natural tick exposure
| Autor: | Susan E. Little, Jeff M. Gruntmeir, Anne W. Barrett, Melissa J. Beall, James H. Meinkoth, Ramaswamy Chandrashekar, Brendon Thatcher, Phyllis Tyrrell, Brett A. Stillman, Lindsay A. Starkey |
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| Rok vydání: | 2014 |
| Předmět: |
Ehrlichia ewingii
Ehrlichia canis Molecular Sequence Data Ehrlichia Enzyme-Linked Immunosorbent Assay Real-Time Polymerase Chain Reaction Microbiology Dogs Ticks parasitic diseases Ehrlichia chaffeensis Animals Dog Diseases Seroconversion General Veterinary biology Base Sequence Ehrlichiosis Oklahoma General Medicine Sequence Analysis DNA biology.organism_classification Virology Antibodies Bacterial Blood Cell Count Canis Ehrlichiosis (canine) Nested polymerase chain reaction Sentinel Surveillance |
| Zdroj: | Veterinary microbiology. 173(3-4) |
| ISSN: | 1873-2542 |
| Popis: | Dogs exposed to ticks in the southern US may become infected with multiple species of Ehrlichia. To better define infection risk, blood samples collected from 10 dogs infested with ticks via a natural infestation model were evaluated by blood smear examination, PCR, patient-side ELISAs (SNAP® 4Dx® and SNAP® 4Dx® Plus), IFA, and peptide based ELISA for evidence of infection with Ehrlichia canis, E. chaffeensis, and/or E. ewingii. Although morulae were rarely identified in blood smears, every dog (10/10) became infected with Ehrlichia spp. as evidenced by nested PCR detection of E. chaffeensis (7/10) and E. ewingii DNA (10/10); real-time PCR detection of E. chaffeensis (0/10) and E. ewingii (9/10); seroconversion on two different patient-side ELISAs (4/10 or 10/10); seroconversion on IFA to E. canis (10/10, maximum inverse titer=128-4096, GMTMAX=548.7) and E. chaffeensis (10/10, maximum inverse titer=1024-32,768, GMTMAX=4096); and seroconversion on peptide specific ELISA to E. chaffeensis VLPT (7/10) and E. ewingii p28 (9/10). Rickettsemia with E. chaffeensis and E. ewingii, as determined by nested PCR, persisted in dogs for an average of 3.2 or 30.5 days, respectively. Ehrlichia canis was not detected in any dog by any method, and no dogs developed signs of clinical disease. Our data suggest that in areas where ticks are common, dogs are at high risk of infection with Ehrlichia spp., particularly E. ewingii and E. chaffeensis, and can serve as a sentinel for monitoring for the presence of these zoonotic pathogens. |
| Databáze: | OpenAIRE |
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