Oxysophoridine suppresses the growth of hepatocellular carcinoma in mice: In Vivo and cDNA microarray studies
| Autor: | Wei Long, Xiao-qing Yao, Pei-Xun Liu, Yun-hui Zhang |
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| Rok vydání: | 2012 |
| Předmět: |
Carcinoma
Hepatocellular genetic structures Microarray Biology Real-Time Polymerase Chain Reaction Weight Gain Mice Oxysophoridine Alkaloids Liver Neoplasms Experimental Osteoprotegerin In vivo Cell Line Tumor Complementary DNA medicine Animals Pharmacology (medical) Cell Proliferation Oligonucleotide Array Sequence Analysis General Medicine medicine.disease Molecular biology digestive system diseases Tumor Burden Gene Expression Regulation Neoplastic Mice Inbred C57BL Real-time polymerase chain reaction Complementary and alternative medicine Organ Specificity Medicine public health Hepatocellular carcinoma Drug Screening Assays Antitumor |
| Zdroj: | Chinese Journal of Integrative Medicine. 18:209-213 |
| ISSN: | 1993-0402 1672-0415 |
| Popis: | To observe the in vivo effects of oxysophoridine on hepatocellular carcinoma in mice and to study the related mechanisms.C57BL mice were inoculated with mouse hepatoma H22 cells subcutaneously, then divided into 5 groups (14 per group), and treated with oxysophoridine (50, 100, or 150 mg/kg) or cisplatin (4 mg/kg) for 10 days. Inhibitory rate of tumor, body weight gain, and influence indices on internal organs (liver, spleen and thymus) were evaluated. The differentially expressed genes between the oxysophoridine-treated group, and the control group were analyzed using cDNA microarray and quantitative real-time PCR (qRT-PCR) experiments.Compared with the tumor weight of the control group (2.75±0.66 g), oxysophoridine significantly suppressed hepatocellular carcinoma growth in mice (P0.01), with 0.82±0.36 g, 0.57±0.22 g, and 1.22±0.67 g for the tumor weight in the low, moderate, and high dose treatment group, respectively. The moderate dose led to the highest inhibitory rate, 79.3%. Observation of body weight gain and influence on three organs showed that compared with cisplatin, oxysophoridine produced fewer side effects in vivo. cDNA microarray and qRT-PCR showed that the most significant differentially expressed genes in the tumor samples of oxysophoridine-treated mice were mostly involved in regulating apoptosis, with the Tnfrsf11b (osteoprotegerin) gene being the most significantly affected.Oxysophoridine was a promising compound for developing drugs against hepatocellular carcinoma, and its anti-hepatoma effect was probably related to osteoprotegerin activation. |
| Databáze: | OpenAIRE |
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