High frequency of Epstein-Barr virus (EBV) lymphoblastoid cell line-reactive lymphocytes in cord blood: evaluation of cytolytic activity and IL-2 production
| Autor: | A. Gasparoni, Francesco Locatelli, M G Revello, G. Mingrat, Patrizia Comoli, Rita Maccario, Alessandro Moretta, Giorgio Rondini, G. Gerna, Daniela Montagna, I. Carena, Elena Percivalle |
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| Rok vydání: | 1997 |
| Předmět: |
Interleukin 2
Adult CD4-Positive T-Lymphocytes Herpesvirus 4 Human medicine.medical_treatment Lymphocyte Immunology Biology Cell Line Immune system hemic and lymphatic diseases medicine Immunology and Allergy Cytotoxic T cell Humans Lymphocytes cord blood lymphocytes cytotoxic activity EBV lymphoblastoid cell lines IL-2 production NK cells Infant Newborn T lymphocyte Original Articles Fetal Blood CD56 Antigen Immunity Innate Killer Cells Natural Cytokine medicine.anatomical_structure Cord blood Interleukin-2 CD8 medicine.drug T-Lymphocytes Cytotoxic |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0009-9104 |
| Popis: | SUMMARY We investigated natural immunity towards autologous EBV lymphoblastoid cell lines (EBV-LCL) in the cord blood. Cord blood lymphocytes (CBL) from 20 healthy neonates were examined together with three EBV+ and one EBV− adult donors. We found that high frequencies of EBV-LCL-reactive cytotoxic lymphocytes, ranging from 1/190 to 1/12 205 were detectable in EBV− and EBV+ donors, as well as in the cord blood of 15 out of 20 neonates. Surface phenotype analysis, depletion experiments with MoAbs specific for T and natural killer (NK) lymphocyte subsets, and T lymphocyte cloning procedures strongly indicate virus-specific cytotoxic T lymphocytes (CTL) as the major population responsible for the lysis of autologous EBV-LCL in EBV+ donors. Conversely, a high frequency of NK cells seems to be involved in the killing activity observed in neonates and in the EBV− donor. Frequencies of EBV-LCL-induced IL-2-producing lymphocytes were high in EBV+ donors (range 1/2247–1/6633) and heterogeneous, but consistent, in cord blood (range 1/5072–1/57 819) and in the EBV− adult (1/17 148). CD8+ lymphocytes were responsible for IL-2 production in EBV+ individuals, while CD4+ T cells were charged with this role in cord blood and in the EBV− donor. These data demonstrate that CBL are able to develop a strong innate immunity, directed against autologous EBV-infected cells, mediated by both NK cells and CD4+ T lymphocytes. This characteristic may be relevant for protection against viral infections in both neonates and patients given cord blood transplantation (CBT). |
| Databáze: | OpenAIRE |
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