Effect of platinum(II) chemotherapeutic agents on properties of DNA liquid crystals
| Autor: | G. Damaschun, H. Damaschun, Yu. M. Yevdokimov, V. I. Salyanov, S. G. Skuridin, V. Kleinwächter, Misselwitz R |
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| Rok vydání: | 1990 |
| Předmět: |
Male
Organoplatinum Compounds Biophysics chemistry.chemical_element Antineoplastic Agents Biochemistry Spectral line chemistry.chemical_compound X-Ray Diffraction Salmon Liquid crystal Animals Molecule Protein secondary structure Chemistry Circular Dichroism Organic Chemistry Polarization Microscopy DNA Spermatozoa Molecular Weight Crystallography X-ray crystallography Cisplatin Crystallization Platinum |
| Zdroj: | Biophysical Chemistry. 35:143-153 |
| ISSN: | 0301-4622 |
| DOI: | 10.1016/0301-4622(90)80004-q |
| Popis: | We have investigated the X-ray and optical properties (CD spectra and polarization microscopy) of liquid-crystalline phases and dispersions formed on pretreatrnent of low molecular weight DNA with the platinum(II) coordination complexes, cis -diammine-dichloroplatinum(II) (DDP), 2,2′ -bipyridinedichloroplatinum(II) ( 1 ) and 2,2′-bipyridineethylenediammineplatinum(II) ( 2 ). It is demonstrated that the platination of DNA leads to the ordering of neighbouring molecules of DNA in liquid-crystalline phases being diminished. The intense bands observed in the CD spectra of liquid-crystalline dispersions prepared from DNA pretreated with 1 or 2 can be used to determine the orientation of the latter compounds with respect to the helical axis of the DNA and to detect distortions in the secondary structure of DNA. The possible causes of the appearance of the intense bands in the CD spectra of liquid-crystalline phases and alterations in the manner of packing of the molecules of DNA within them are discussed. |
| Databáze: | OpenAIRE |
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