| Autor: |
Wen-Xiang Ji, Ye-Chao Tian, Min-Hui Cai, Bi-Cun Jiang, Shi Cheng, Yan Li, Qing Zhou, Bo-Qiang Li, Bai-Yang Chen, Xing Zheng, Wen-Tao Li, Ai-Min Li |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Journal of environmental sciences (China). 125 |
| ISSN: |
1001-0742 |
| Popis: |
Accurate quantification of dissolved organic nitrogen (DON) has been a challenge due to the cumulative analytical errors in the conventional method via subtracting dissolved inorganic nitrogen species (DIN) from total dissolved nitrogen (TDN). Size exclusion chromatography coupled with an organic nitrogen detector (SEC-OND) has been developed as a direct method for quantification and characterization of DON. However, the applications of SEC-OND method still subject to poor separations between DON and DIN species and unsatisfied N recoveries of macromolecules. In this study, we packed a series of SEC columns with different lengths and resin materials for separation of different N species and designed an independent vacuum ultraviolet (VUV) oxidation device for complete oxidation converting N species to nitrate. To guarantee sufficient N recoveries, the operation conditions were optimized as oxidation time ≥ 30 min, injection mass (sample concentration × injection volume)1000 µL × mg-N/L for macromolecular proteins, and neutral pH mobile eluent. The dissolved O |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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