Changes in neurofilament 200 and tyrosine hydroxylase expression in the cardiac innervation of diabetic rats during aging
| Autor: | Marija Bakovic, Livia Puljak, Katarina Vukojević, Marija Vitlov Uljević, Sandra Kostic, Nenad Kunac, Lejla Ferhatović Hamzić, Elena Zdrilic, Natalija Filipović |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Aging Neurofilament Tyrosine 3-Monooxygenase Diabetic Cardiomyopathies Cardiomyopathy Sensory system Pathology and Forensic Medicine Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Neurofilament Proteins Internal medicine Diabetes mellitus medicine Animals Tyrosine hydroxylase business.industry Myocardium Heart General Medicine medicine.disease Streptozotocin Rats 030104 developmental biology medicine.anatomical_structure Endocrinology Diabetes Mellitus Type 1 diabetes mellitus innervation neurofilament 200kDa tyrosine hydroxylase neuropathy Heart failure Cardiology and Cardiovascular Medicine business 030217 neurology & neurosurgery medicine.drug Sensory nerve |
| Zdroj: | Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology. 32 |
| ISSN: | 1879-1336 |
| Popis: | Changes in sensory and sympathetic innervation during diabetes mellitus (DM) can be a predictor of arrhythmias, silent myocardial ischemia, and chronic heart failure, but knowledge about these changes is still unsatisfactory. We analyzed whether prolonged DM induces changes in density of sensory and sympathetic nerve terminals of rat's heart and whether it contributes to cardiomyopathy during aging. DM was induced by i/p injecting 55 mg/kg streptozotocin to male Sprague-Dawley rats, while a control group received a citrate buffer. DM in the rats was validated by measuring blood glucose level. Animals were sacrificed after 2 weeks, 2 months, 6 months, and 12 months. Five areas of cardiac sections were analyzed. Antibodies raised against tyrosine hydroxylase (TH) and neurofilament 200 kDa (NF 200) were used to detect sympathetic and sensory fibers. TH immunoreactive fiber density increased in DM groups 2 weeks after induction, reaching a peek after 2 months, while in the later stages of DM (6 and 12 months), there was no significant difference compared to control. NF 200 immunoreactive fiber density increased 2 weeks after induction compared to control. There was no consistent pattern of change during the given period in both the DM or control groups. In the DM group, we found thickening of the left ventricle wall (P |
| Databáze: | OpenAIRE |
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