Angiotensin converting enzyme DD genotype is associated with development of rheumatic heart disease in Egyptian children
| Autor: | Mohamed-mofeed Fawaz Morsy, Ahmed Mohamed Boghdady, Mohamed Ali Ismail, Hydi Ahmed, Essam Mohamed Abu Elfadl, Nada Abdelmohsen Mohamed Abdelaziz |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Genotype Immunology Biology Peptidyl-Dipeptidase A Polymerase Chain Reaction Polymorphism Single Nucleotide White People chemistry.chemical_compound Rheumatology Gene Frequency Polymorphism (computer science) Immunology and Allergy Humans Genetic Predisposition to Disease Allele Child Allele frequency Gene Alleles Chi-Square Distribution Rheumatic Heart Disease Angiotensin-converting enzyme Molecular biology chemistry biology.protein Egypt Female Gene polymorphism Ethidium bromide |
| Zdroj: | Rheumatology international. 31(1) |
| ISSN: | 1437-160X |
| Popis: | Angiotensin converting enzyme (ACE) gene polymorphism was previously studied in some cardiovascular diseases. There are only few studies which investigated this polymorphism in patients with rheumatic heart disease (RHD). The results of these investigations are inconsistent. Furthermore, gene polymorphism distribution is different in various ethnic populations. We conducted this study to demonstrate this gene polymorphism in Egyptian children with RHD. Leukocytes DNA was extracted from 139 patients with RHD and 79 healthy control children. After amplification by the PCR, the products were separated by electrophoresis in 6% polyacrylamide gel and visualized after ethidium bromide staining with UV light. The PCR product is a 190-bp fragment in the absence of the insertion (D allele) and a 490-bp fragment in the presence of the insertion (I allele). Gene polymorphism was as follows: DD gene when lane contains only 190-bp fragment, II gene when lane contains only 490-bp fragment and ID gene when lane contains both fragments. We found that gene polymorphism in both control and patients groups followed the following order of distribution from highest to lowest: ID, II, DD gene. The frequency in control group was 49.4, 36.7, and 13.9%, respectively. In patients groups, the gene frequency was 42.5, 30.9, and 26.6%, respectively. DD gene frequency differs significantly between the two groups. We concluded that patients with RHD have a higher ACE-DD genotype than normal control. ACE-DD genotype may be a risk factor for RHD in Egyptian children. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink