Differential effects of allergen challenge on large and small airway reactivity in mice
| Autor: | Mimi L.K. Tang, Simon G. Royce, Jane E. Bourke, Chantal Donovan, James Esposito, Jenny Tran, Zaridatul Aini Ibrahim, Simon R. Bailey |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Ovalbumin
lcsh:Medicine Bronchial Provocation Tests Dinoprostone 03 medical and health sciences Mice 0302 clinical medicine Airway resistance In vivo Antibody Specificity Medicine Animals Respiratory system lcsh:Science Methacholine Chloride 030304 developmental biology Asthma Inflammation 0303 health sciences Multidisciplinary Lung biology business.industry lcsh:R Allergens Immunoglobulin E respiratory system medicine.disease respiratory tract diseases Disease Models Animal medicine.anatomical_structure 030228 respiratory system Immunology biology.protein Airway Remodeling Methacholine Calcium Female lcsh:Q Bronchial Hyperreactivity Airway business medicine.drug Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 9, p e74101 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The relative contributions of large and small airways to hyperresponsiveness in asthma have yet to be fully assessed. This study used a mouse model of chronic allergic airways disease to induce inflammation and remodelling and determine whether in vivo hyperresponsiveness to methacholine is consistent with in vitro reactivity of trachea and small airways. Balb/C mice were sensitised (days 0, 14) and challenged (3 times/week, 6 weeks) with ovalbumin. Airway reactivity was compared with saline-challenged controls in vivo assessing whole lung resistance, and in vitro measuring the force of tracheal contraction and the magnitude/rate of small airway narrowing within lung slices. Increased airway inflammation, epithelial remodelling and fibrosis were evident following allergen challenge. In vivo hyperresponsiveness to methacholine was maintained in isolated trachea. In contrast, methacholine induced slower narrowing, with reduced potency in small airways compared to controls. In vitro incubation with IL-1/TNFα did not alter reactivity. The hyporesponsiveness to methacholine in small airways within lung slices following chronic ovalbumin challenge was unexpected, given hyperresponsiveness to the same agonist both in vivo and in vitro in tracheal preparations. This finding may reflect the altered interactions of small airways with surrounding parenchymal tissue after allergen challenge to oppose airway narrowing and closure. |
| Databáze: | OpenAIRE |
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