Rotaviruses: Extraction and Isolation of RNA, Reassortant Strains, and NSP4 Protein
| Autor: | Zachary D. Franklin, Judith M. Ball, Krystle A. Yakshe |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Rotavirus
Insecta viruses Biology Viral Nonstructural Proteins medicine.disease_cause Microbiology Genome law.invention Cell Line law Virology medicine Animals Typing Gene Glycoproteins Toxins Biological Mammals RNA General Medicine Isolation (microbiology) biology.protein Recombinant DNA RNA Viral Parasitology Antibody Reassortant Viruses |
| Zdroj: | Current Protocols in Microbiology |
| ISSN: | 1934-8533 |
| Popis: | Rotavirus (RV) contains 11 double-stranded RNA segments that encode for twelve structural and nonstructural proteins. The separation and isolation of viral RNA is a necessary precursor for many experimental techniques and can be useful for rapid RV RNA typing and sequencing of different rotavirus strains. The segmented genome enables RV to recombine easily. These recombinant viruses are essential for many purposes, including generation of potential vaccine strains. Rotavirus gene 10 expresses the viral enterotoxin, NSP4, which has been the focus of several studies due to the influence of NSP4 on rotavirus replication, morphogenesis, and pathogenesis. This unit will describe the isolation and separation of viral RNAs, the production characterization of recombinant RV in culture, and the expression and isolation of NSP4 in mammalian and insect cells. © 2015 by John Wiley & Sons, Inc. Keywords: rotavirus; reassortants; RNA electrophoresis; NSP4; peptide antibodies; chromatography |
| Databáze: | OpenAIRE |
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