Easy identification of leishmania species by mass spectrometry
| Autor: | Pierre Buffet, Gloriat Morizot, Eric Caumes, Christophe Ravel, Nathalie Chartrel, Oussama Mouri, Stéphane Jauréguiberry, Marc Thellier, Gert Van der Auwera, Marie Passet, Carine Marinach-Patrice, Isabelle Joly, Dominique Mazier |
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| Přispěvatelé: | HAL UPMC, Gestionnaire, Service de Parasitologie - Mycologie [CHU Pitié-Salpétrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Immunologie moléculaire des parasites, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institute of Tropical Medicine [Antwerp] (ITM), Centre National de Référence des Leishmanioses [CHRU Montpellier] (CNR-L), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Université de Montpellier (UM), Université Pierre et Marie Curie - Paris 6 (UPMC), Immunité et Infection, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR113-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Maladies Infectieuses et Tropicales [CHU Pitié-Salpêtrière], Service de parasitologie - mycologie [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR113-Université Pierre et Marie Curie - Paris 6 (UPMC), Service des maladies infectieuses et tropicales [CHU Pitié-Salpêtrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU) |
| Rok vydání: | 2013 |
| Předmět: |
Male
Proteomics Time Factors Medical Physics Veterinary Microbiology Global Health Biochemistry Pediatrics Medicine and Health Sciences Leishmania major Public and Occupational Health Child Genetics Aged 80 and over Leishmania Travel [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology biology lcsh:Public aspects of medicine Systems Biology Physics Middle Aged 3. Good health Infectious Diseases Veterinary Diseases Research Design Physical Sciences Female Synthetic Biology France Leishmania infantum Subgenus Research Article Adult Veterinary Medicine Species complex lcsh:Arctic medicine. Tropical medicine Adolescent lcsh:RC955-962 Clinical Research Design Immunology Leishmania donovani Biophysics Leishmaniasis Cutaneous Dermatology Research and Analysis Methods Microbiology Young Adult Cutaneous leishmaniasis Diagnostic Medicine medicine Humans Aged Evolutionary Biology Clinical Laboratory Techniques Public Health Environmental and Occupational Health Biology and Life Sciences Leishmaniasis lcsh:RA1-1270 biology.organism_classification medicine.disease Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Multilocus sequence typing Parasitology Clinical Immunology Veterinary Science Clinical Medicine [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Developmental Biology |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, 2014, 8 (6), pp.e2841. ⟨10.1371/journal.pntd.0002841⟩ PLoS Neglected Tropical Diseases, Public Library of Science, 2014, 8 (6), pp.e2841. ⟨10.1371/journal.pntd.0002841⟩ PLoS Neglected Tropical Diseases, Vol 8, Iss 6, p e2841 (2014) |
| ISSN: | 1935-2735 1935-2727 |
| Popis: | Background Cutaneous leishmaniasis is caused by several Leishmania species that are associated with variable outcomes before and after therapy. Optimal treatment decision is based on an accurate identification of the infecting species but current methods to type Leishmania isolates are relatively complex and/or slow. Therefore, the initial treatment decision is generally presumptive, the infecting species being suspected on epidemiological and clinical grounds. A simple method to type cultured isolates would facilitate disease management. Methodology We analyzed MALDI-TOF spectra of promastigote pellets from 46 strains cultured in monophasic medium, including 20 short-term cultured isolates from French travelers (19 with CL, 1 with VL). As per routine procedure, clinical isolates were analyzed in parallel with Multilocus Sequence Typing (MLST) at the National Reference Center for Leishmania. Principal Findings Automatic dendrogram analysis generated a classification of isolates consistent with reference determination of species based on MLST or hsp70 sequencing. A minute analysis of spectra based on a very simple, database-independent analysis of spectra based on the algorithm showed that the mutually exclusive presence of two pairs of peaks discriminated isolates considered by reference methods to belong either to the Viannia or Leishmania subgenus, and that within each subgenus presence or absence of a few peaks allowed discrimination to species complexes level. Conclusions/Significance Analysis of cultured Leishmania isolates using mass spectrometry allows a rapid and simple classification to the species complex level consistent with reference methods, a potentially useful method to guide treatment decision in patients with cutaneous leishmaniasis. Author Summary Cutaneous leishmaniasis is a disease due to a small parasite called Leishmania. This parasite causes disfiguring skin lesions that last for months or years. There are many different subtypes of Leishmania, each giving rise to lesions of different severity and responding to therapies in its own way. Treating physicians must know as soon as possible which subtype of Leishmania is involved to propose the best treatment. Because it is impossible to differentiate the Leishmania subtypes microscopically, the identification of the culprit subtype currently requires complex and expensive typing methods, the results of which are generally obtained several weeks after the diagnosis. Here, we have evaluated the ability of a new method using mass spectrometry to differentiate Leishmania subtypes. Our results were consistent with those provided by reference typing methods and were obtained rapidly after the parasite had been cultured in vitro. This new method may help physicians know very soon which Leishmania subtype is involved thereby facilitating treatment choice. |
| Databáze: | OpenAIRE |
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