miR-4306 Suppresses Proliferation of Esophageal Squamous Cell Carcinoma Cell by Targeting SIX3
| Autor: | Xiaoxia Wang, Chengyuan Yang, Zichan Guo, Yongmei Song, Zitong Zhao, Yuan Wei |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cisplatin 030102 biochemistry & molecular biology Cell growth Cell Biophysics Cell Biology General Medicine Tumor initiation Biology medicine.disease Biochemistry Phenotype digestive system diseases 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Breast cancer Cell culture Apoptosis medicine Cancer research Esophageal Squamous Cell Carcinoma medicine.drug |
| Zdroj: | Cell biochemistry and biophysics. 79(4) |
| ISSN: | 1559-0283 |
| Popis: | Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers and the primary cause of cancer-related mortality in China. micoRNA plays a vital role during tumor initiation and malignant progression. miR-4306 has been reported to negatively regulate aggressive cell phenotypes in triple-negative breast cancer (TNBC). Nevertheless, the function of miR-4306 in ESCC was still not clear. In this study, we detected miR-4306 expression by quantitative real-time reverse transcription-PCR (qRT-PCR) and found that miR-4306 expression was downregulated in human ESCC tissue samples and cell lines. Moreover, miR-4306 overexpression could restrain ESCC cell proliferation, migratory and invasive ability and epithelial-mesenchymal transition (EMT), promote cell apoptosis after treatment with or without cisplatin. In contrast, inhibiting the expression of miR-4306 showed the opposing results. Furthermore, we explored the molecular mechanism of effects of miR-4306 and found that miR-4306 inhibited the expression of SIX3 by interaction with SIX3 3'UTR in ESCC cells, and SIX3 overexpression significantly reversed the effect of miR-4306-mediated ESCC cells proliferation. The current study provided evidence of miR-4306 as a tumor suppression gene in ESCC. |
| Databáze: | OpenAIRE |
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