Changes in lipopolysaccharide profile of Porphyromonas gingivalis clinical isolates correlate with changes in colony morphology and polymyxin B resistance
| Autor: | Jorge Gamonal, Anilei Hoare, Nora Silva, Isaac Bugueño, Daniela Salinas, Cristopher Soto, Leonor Díaz, Nicolas Dutzan, José M. Pérez-Donoso, Denisse Bravo, Darna Venegas |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Lipopolysaccharides Male Cell Membrane Permeability Membrane permeability Virulence Factors Virulence Microbial Sensitivity Tests Biology Microbiology Young Adult RNA Ribosomal 16S Drug Resistance Bacterial Bacteroidaceae Infections medicine Humans Ligase activity Periodontitis Porphyromonas gingivalis Novobiocin Polymyxin B Middle Aged medicine.disease biology.organism_classification Anti-Bacterial Agents Infectious Diseases Genes Bacterial Interaction with host Case-Control Studies Female medicine.drug |
| Zdroj: | Anaerobe. 33:25-32 |
| ISSN: | 1075-9964 |
| DOI: | 10.1016/j.anaerobe.2015.01.009 |
| Popis: | Virulence factors on the surface of Porphyromonas gingivalis constitute the first line of interaction with host cells and contribute to immune modulation and periodontitis progression. In order to characterize surface virulence factors present on P. gingivalis , we obtained clinical isolates from healthy and periodontitis subjects and compared them with reference strains. Colony morphology, aggregation in liquid medium, surface charge, membrane permeability to bactericidal compounds, novobiocin and polymyxin B resistance, capsule presence and lipopolysaccharide (LPS) profiles were evaluated. By comparing isolates from healthy and periodontitis subjects, differences in colony morphology and aggregation in liquid culture were found; the latter being similar to two reference strains. These differences were not a consequence of variations in bacterial surface charge. Furthermore, isolates also presented differences in polymyxin B and novobiocin resistance; isolates from healthy subjects were susceptible to polymyxin B and resistant to novobiocin and, in contrast, isolates from periodontitis subjects were resistant to polymyxin B and susceptible to novobiocin. These changes in antimicrobial resistance levels correlate with variations in LPS profiles, since -unlike periodontitis isolates–isolates from healthy samples synthesize LPS molecules lacking both O-antigen moieties and anionic polysaccharide. Additionally, this phenotype correlated with the absence of O-antigen ligase activity. Altogether, our results reveal novel variations on surface components of P. gingivalis isolates obtained from healthy and periodontitis subjects that could be associated with differences in bacterial virulence and periodontitis progression. |
| Databáze: | OpenAIRE |
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