Heparan Sulfates Support Pyramidal Cell Excitability, Synaptic Plasticity, and Context Discrimination
| Autor: | Arie Oosterhof, Michel K. Herde, Christian Henneberger, Alexander Dityatev, Toin H. van Kuppevelt, Andreas B. Wulff, Gaga Kochlamazashvili, Olga Tikhobrazova, Oleg Senkov, Daniel Minge, Andrey Mironov, Rahul Kaushik |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Long-Term Potentiation biosynthesis [Ankyrins] extracellular matrix heparan sulfates synaptic plasticity neuronal excitability learning Mice Discrimination Psychological 0302 clinical medicine Conditioning Psychological LTP induction physiology [Neuronal Plasticity] Fear conditioning Theta Rhythm physiology [Heparitin Sulfate] Neuronal Plasticity physiology [Pyramidal Cells] Chemistry Pyramidal Cells Long-term potentiation Fear genetics [Ankyrins] CA3 Region Hippocampal cytology [CA3 Region Hippocampal] physiology [CA3 Region Hippocampal] physiology [Discrimination Psychological] medicine.anatomical_structure Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] Ank3 protein mouse physiology [Calcium Signaling] physiology [Nerve Net] Pyramidal cell Ankyrins Cognitive Neuroscience In Vitro Techniques Neurotransmission 03 medical and health sciences Cellular and Molecular Neuroscience medicine Animals Calcium Signaling ddc:610 CA1 Region Hippocampal pharmacology [Heparin Lyase] physiology [CA1 Region Hippocampal] cytology [CA1 Region Hippocampal] Original Articles Axon initial segment Mice Inbred C57BL 030104 developmental biology Heparin Lyase nervous system Schaffer collateral Synapses Synaptic plasticity Heparitin Sulfate Nerve Net physiology [Long-Term Potentiation] physiology [Synapses] Neuroscience physiology [Fear] 030217 neurology & neurosurgery |
| Zdroj: | Cerebral Cortex, 27, 2, pp. 903-918 Cerebral cortex, 27(2): 903-918 Cerebral Cortex, 27, 903-918 Cerebral cortex 27(2), cercor;bhx003v1 (2017). doi:10.1093/cercor/bhx003 Cerebral Cortex (New York, NY) |
| ISSN: | 1047-3211 |
| DOI: | 10.1093/cercor/bhx003 |
| Popis: | Contains fulltext : 174419.pdf (Publisher’s version ) (Open Access) Heparan sulfate (HS) proteoglycans represent a major component of the extracellular matrix and are critical for brain development. However, their function in the mature brain remains to be characterized. Here, acute enzymatic digestion of HS side chains was used to uncover how HSs support hippocampal function in vitro and in vivo. We found that long-term potentiation (LTP) of synaptic transmission at CA3-CA1 Schaffer collateral synapses was impaired after removal of highly sulfated HSs with heparinase 1. This reduction was associated with decreased Ca2+ influx during LTP induction, which was the consequence of a reduced excitability of CA1 pyramidal neurons. At the subcellular level, heparinase treatment resulted in reorganization of the distal axon initial segment, as detected by a reduction in ankyrin G expression. In vivo, digestion of HSs impaired context discrimination in a fear conditioning paradigm and oscillatory network activity in the low theta band after fear conditioning. Thus, HSs maintain neuronal excitability and, as a consequence, support synaptic plasticity and learning. |
| Databáze: | OpenAIRE |
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